Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Fibrinogen-binding protein/clumping factor from Staphylococcus aureus.

M K Bodén1, J I Flock

  • 1Center for Biotechnology, Karolinska Institute, Huddinge, Sweden.

Infection and Immunity
|August 1, 1989
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Lack of fbe, the gene for a fibrinogen-binding protein from Staphylococcus epidermidis, reduces its adherence to fibrinogen coated surfaces.

Microbial pathogenesis·2001
Same author

Extracellular fibrinogen-binding protein, Efb, from Staphylococcus aureus blocks platelet aggregation due to its binding to the alpha-chain.

The Journal of biological chemistry·2001
Same author

Functional study of antibodies against a fibrogenin-binding protein in Staphylococcus epidermidis adherence to polyethylene catheters.

The Journal of infectious diseases·2001
Same author

Rebinding of extracellular adherence protein Eap to Staphylococcus aureus can occur through a surface-bound neutral phosphatase.

Journal of bacteriology·2001
Same author

Antibodies against a truncated Staphylococcus aureus fibronectin-binding protein protect against dissemination of infection in the rat.

Vaccine·2001
Same author

Collagen and fibronectin binding in experimental staphylococcal osteomyelitis.

Clinical orthopaedics and related research·2001
Same journal

The cholesterol-dependent cytolysin promotes <i>Streptococcus</i> systemic spread and induces arachidonic acid accumulation-mediated lethality in a murine intraperitoneal infection model.

Infection and immunity·2026
Same journal

Phenotypic and genotypic analysis of <i>Candida albicans</i> vaginal isolates reveals that <i>ECE1</i> expression underpins pathogenicity.

Infection and immunity·2026
Same journal

<i>Staphylococcus aureus</i> pore-forming toxins differentially shape disease severity in experimental endophthalmitis.

Infection and immunity·2026
Same journal

Group B streptococcal membrane vesicles induce proinflammatory responses in neonatal meninges.

Infection and immunity·2026
Same journal

<i>Pseudomonas aeruginosa</i> infection causes lysosomal dysfunction in the cystic fibrosis bronchial epithelium.

Infection and immunity·2026
Same journal

The role of probiotics in restoring and maintaining vaginal microbiome health: a review.

Infection and immunity·2026
See all related articles

Staphylococcal coagulase binds to fibrinogen, mediating bacterial clumping. This study identifies key fibrinogen-binding proteins, including coagulase, and confirms their role in plasma clot formation.

Area of Science:

  • Microbiology
  • Biochemistry

Background:

  • Staphylococcal infections are a significant health concern.
  • Understanding bacterial adherence and virulence factors is crucial for developing effective treatments.

Purpose of the Study:

  • To investigate the interaction between staphylococcal components and fibrinogen.
  • To identify and characterize the specific staphylococcal molecules responsible for fibrinogen binding.

Main Methods:

  • Affinity purification of staphylococcal components using fibrinogen-Sepharose.
  • Western blotting with specific antibodies to detect fibrinogen-binding proteins.
  • Plasma coagulase tests to assess clotting activity.
  • Surface labeling with radioactive iodine (125I) to determine cell surface localization.

Related Experiment Videos

Main Results:

  • Two major fibrinogen-binding bands (87 kDa and 19 kDa) and a minor band (35 kDa) were identified.
  • The 87 kDa band was confirmed as coagulase, exhibiting plasma clotting activity and binding to prothrombin.
  • Both 87 kDa and 19 kDa molecules were found on the staphylococcal cell surface.
  • Extracellular products yielded higher amounts of these binding molecules compared to washed cells.

Conclusions:

  • Staphylococcal coagulase binds to fibrinogen, contributing to bacterial clumping.
  • Cell-bound coagulase retains affinity for fibrinogen independently of prothrombin.
  • These findings elucidate a key mechanism in staphylococcal pathogenesis and host interaction.