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Updated: Mar 17, 2026
Biosensor for Detection of Antibiotic Resistant Staphylococcus Bacteria
Published on: May 8, 2013
Antibiotic resistance patterns of Staphylococcus aureus: A multi center study from India
Suresh Kumar Mendem1, Triveni Alasthimannahalli Gangadhara1, Channappa T Shivannavar1
1Department of P. G Studies and Research in Microbiology, Gulbarga University, Kalaburagi, 585 106, Karnataka, India.
Abstract:
Chemotherapy and emergence of drug resistance strains of Staphylococcus aureus is receiving serious threats, due to the origin and spread of hospital and community acquired MDR strains. The present study reports the prevalence of antibiotic resistance among Staphylococcus aureus isolated from clinical samples from different cities of India. Antibiotic sensitivity was performed by Kirby-Bauer disk diffusion method and minimum inhibitory concentrations were determined for vancomycin and methicillin according to CLSI (2014) guidelines. A total of 212 S. aureus were obtained from different samples such as pus, blood, urine. The antibiogram of these isolates indicated widespread resistance to various groups of antibiotics ranging from a minimum of 10.13% against Phenicols (Chloramphenicol) to a maximum of 97% against Penicillin and 44.8% isolates were MRSA and alarmingly 10.84% were VRSA. Most of the MRSA isolates showed inducible Clindamycin resistance. Widespread prevalence of MDR patterns, increasing incidence of MRSA and VRSA calls for exploration of alternative medicines and new approaches to combat Staphylococcal infections.
Insights
Antibiotic resistance in Staphylococcus aureus is a growing threat in India. This study found high rates of Methicillin-resistant Staphylococcus aureus (MRSA) and Vancomycin-resistant Staphylococcus aureus (VRSA), highlighting the urgent need for new treatments.
Area of Science:
- Microbiology
- Infectious Diseases
- Pharmacology
Background:
- The rise of multidrug-resistant (MDR) strains of Staphylococcus aureus, including hospital and community-acquired strains, poses a significant global health threat.
- Antibiotic resistance in Staphylococcus aureus necessitates ongoing surveillance and research into effective treatment strategies.
Purpose of the Study:
- To investigate the prevalence of antibiotic resistance patterns in Staphylococcus aureus isolated from clinical samples across various Indian cities.
- To determine the incidence of Methicillin-resistant Staphylococcus aureus (MRSA) and Vancomycin-resistant Staphylococcus aureus (VRSA) in these isolates.
Main Methods:
- Antibiotic sensitivity testing was conducted using the Kirby-Bauer disk diffusion method.
- Minimum inhibitory concentrations (MICs) for vancomycin and methicillin were determined following Clinical and Laboratory Standards Institute (CLSI) 2014 guidelines.
- Staphylococcus aureus isolates were obtained from clinical samples including pus, blood, and urine.
Main Results:
- Out of 212 Staphylococcus aureus isolates, widespread resistance was observed, with resistance rates ranging from 10.13% for Chloramphenicol to 97% for Penicillin.
- A significant proportion of isolates were identified as MRSA (44.8%) and VRSA (10.84%).
- A majority of MRSA isolates exhibited inducible Clindamycin resistance.
Conclusions:
- The high prevalence of MDR, MRSA, and VRSA strains of Staphylococcus aureus in India underscores a critical public health challenge.
- There is an urgent need to explore alternative medicines and novel therapeutic approaches to effectively combat Staphylococcus aureus infections.
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