Related Experiment Videos
Detection of furazolidone in human biological fluids by high performance liquid chromatography
A Valadez-Salazar1, H Guiscafre-Gallardo, S Sanchez-Garcia
1Hospital de Pediatria, Centro Medico Nacional, México, D.F.
Abstract:
Furazolidone has normally been administered as a non-absorbable antimicrobial agent for use in gastrointestinal infections. However, in India and Mexico it has been used successfully for the treatment of typhoid fever. We measured concentrations of furazolidone by high performance liquid chromatography (HPLC) in several biological fluids, after a single oral dose (5 mg/kg). Six healthy adult volunteers and seven children with typhoid fever and ten children with purulent meningitis were studied. In adults the peak serum concentration was less than or equal to 0.84 mg/l and less than or equal to 4.78% of the ingested dose was excreted in the urine. In the children concentrations were similar to those found in volunteers. The cerebrospinal fluid/serum ratio ranged from 1.02 to 5.95 in the meningitis patients. HPLC is a rapid and sensitive method for the quantitation of furazolidone in biological fluids. The minimum detectable concentration was 0.05 mg/l, with a precision of 6% from peak area and an average recovery of 98%.
Insights
Furazolidone, an antimicrobial, shows promising absorption and distribution in the body, including cerebrospinal fluid, suggesting potential for treating infections like typhoid fever.
Area of Science:
- Pharmacology
- Clinical Microbiology
- Analytical Chemistry
Background:
- Furazolidone is typically a non-absorbable antimicrobial for gastrointestinal infections.
- It has been successfully used for typhoid fever treatment in India and Mexico.
Purpose of the Study:
- To measure furazolidone concentrations in biological fluids after oral administration.
- To evaluate its pharmacokinetic profile in healthy adults and children with infections.
Main Methods:
- High-performance liquid chromatography (HPLC) was used for furazolidone quantification.
- Single oral dose of 5 mg/kg was administered to volunteers and patients.
- Biological fluids analyzed included serum, urine, and cerebrospinal fluid.
Main Results:
- Peak serum furazolidone concentrations in adults were ≤0.84 mg/l.
- Urinary excretion in adults was ≤4.78% of the ingested dose.
- Cerebrospinal fluid/serum ratios in meningitis patients ranged from 1.02 to 5.95.
Conclusions:
- HPLC is a rapid and sensitive method for furazolidone quantitation (MDQ 0.05 mg/l).
- Furazolidone demonstrates absorption and distribution into cerebrospinal fluid.
- Findings support its potential therapeutic use beyond gastrointestinal infections.