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Oleic acid reduces pulmonary microvascular sieving capacity in sheep
L Smith1, S Andreasson, S Bergdahl
1Department of Surgery I, University of Göteborg, Sweden.
Journal of Applied Physiology (Bethesda, Md. : 1985)
|June 1, 1989
Summary
Oleic acid injection increases pulmonary microvascular permeability in sheep by enlarging and increasing the number of large pores in the vessel walls. This study quanties changes in osmotic reflection coefficients and pore sizes.
Area of Science:
- Pulmonary physiology
- Vascular biology
- Microcirculation research
Background:
- Pulmonary microvascular permeability is crucial for lung function.
- Oleic acid is a model agent used to induce acute lung injury.
- Understanding changes in vascular permeability is key to treating lung conditions.
Purpose of the Study:
- To investigate the effects of oleic acid on pulmonary microvascular permeability in sheep.
- To quantify changes in osmotic reflection coefficients for various proteins.
- To determine alterations in the size and number of microvascular pores.
Main Methods:
- Chronic lung fistula preparation in sheep.
- Measurement of osmotic reflection coefficients (sigma d) for total protein, albumin, IgG, and IgM.
- Calculation of equivalent small and large pore radii before and after oleic acid infusion.
Main Results:
- Oleic acid significantly decreased sigma d values for all proteins studied.
- The radius of large pores increased from 225 A to 275 A.
- The number of large pores increased by 195% after oleic acid infusion.
- Arterial pressure and oxygen tension decreased, with leukocyte and platelet consumption.
Conclusions:
- Oleic acid induces a substantial increase in pulmonary microvascular permeability.
- This increase is primarily attributed to an enhanced size and number of large pores.
- The findings provide insights into the mechanisms of oleic acid-induced lung injury.