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Immunoprecipitation of ROR1
Vincent T Bicocca1, Jeffrey W Tyner2
1Hematology and Medical Oncology, Oregon Health and Science University, Portland, USA.
Bio-Protocol
|July 26, 2016
Summary
Researchers developed a new protocol to isolate ROR1, a protein linked to cancer, from specific leukemia cell lines. This method aids in studying ROR1’s role in t(1;19) acute lymphoblastic leukemia.
Area of Science:
- Oncology
- Molecular Biology
- Biochemistry
Background:
- ROR1 is a receptor tyrosine kinase involved in cellular signaling.
- ROR1 plays a role in both embryonic development and cancer progression.
- The t(1;19) chromosomal translocation is a specific subtype of acute lymphoblastic leukemia.
Purpose of the Study:
- To establish a reliable method for isolating endogenous ROR1.
- To facilitate further research into ROR1’s function in t(1;19) acute lymphoblastic leukemia.
Main Methods:
- Immunoprecipitation technique.
- Utilized immortalized cell lines from t(1;19) acute lymphoblastic leukemia patients.
- Focused on isolating endogenous ROR1 protein.
Main Results:
- A protocol for immunoprecipitation of endogenous ROR1 was successfully described.
- The method is applicable to specific leukemia cell line models.
Conclusions:
- The described protocol provides a tool for investigating ROR1 in the context of t(1;19) acute lymphoblastic leukemia.
- Further studies can utilize this method to explore ROR1's contribution to this specific cancer subtype.
