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Flow Cytometry

Flow Cytometry

The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
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Related Experiment Video

Updated: Mar 17, 2026

Orthotopic Implantation and Peripheral Immune Cell Monitoring in the II-45 Syngeneic Rat Mesothelioma Model

Orthotopic Implantation and Peripheral Immune Cell Monitoring in the II-45 Syngeneic Rat Mesothelioma Model

Published on: October 2, 2015

ROR1 Flow Cytometry.

Vincent T Bicocca¹, Jeffrey W Tyner²

¹Hematology and Medical Oncology, Oregon Health and Science University, Portland, USA.

|July 26, 2016

Summary

This summary is machine-generated.

This study presents a flow cytometry protocol to measure ROR1 protein on the surface of acute lymphoblastic leukemia cells. This method aids in understanding ROR1

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Last Updated: Mar 17, 2026

Orthotopic Implantation and Peripheral Immune Cell Monitoring in the II-45 Syngeneic Rat Mesothelioma Model

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Published on: October 2, 2015

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Analysis of SAMHD1 Restriction by Flow Cytometry in Human Myeloid U937 Cells

Analysis of SAMHD1 Restriction by Flow Cytometry in Human Myeloid U937 Cells

Published on: June 13, 2021

Area of Science:

Oncology
Cell Biology
Biochemistry

Background:

Receptor tyrosine kinase like orphan receptor 1 (ROR1) is implicated in cancer progression and embryonic development.
ROR1's role in acute lymphoblastic leukemia (ALL) necessitates reliable methods for its assessment.

Purpose of the Study:

To establish and validate a flow cytometry protocol for quantifying ROR1 surface expression.
To provide a standardized method for ROR1 analysis in ALL cell lines.

Main Methods:

Utilizing immortalized acute lymphoblastic leukemia cell lines.
Employing flow cytometry for cell surface protein detection.
Developing a specific staining and analysis protocol for ROR1.

Main Results:

A robust protocol for ROR1 surface expression analysis was successfully developed.
The protocol allows for quantitative assessment of ROR1 in ALL cell line models.

Conclusions:

The described flow cytometry protocol is effective for analyzing ROR1 expression in ALL.
This method can facilitate further research into ROR1's function in leukemia.