Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Overview Of Cell Separation And Isolation

Overview Of Cell Separation And Isolation

Cell separation was first achieved in 1964 by S. H. Seal, who separated large tumor cells from the smaller blood cells using filtration. Two years later, Pohl and Hawk performed experiments on how cells respond differently to a nonuniform electric field based on the cell type. Such observations were the inception of cell separation methods, which allow isolating a single cell type from a heterogeneous sample.

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

PERK Deficiency Amplifies Molecular, Structural, and Network Vulnerability to Repetitive Mild Traumatic Brain Injury.

bioRxiv : the preprint server for biology·2026

Same author

The loss of Tau in the adult brain triggers neuroplastic, epigenetic and behavioral deficits.

bioRxiv : the preprint server for biology·2026

Same author

Spatial mapping of the brain metabolome lipidome and glycome.

Nature communications·2025

Same author

Age dictates brain functional connectivity and axonal integrity following repetitive mild traumatic brain injuries in mice.

NeuroImage·2024

Same author

Age dictates brain functional connectivity and axonal integrity following repetitive mild traumatic brain injuries.

bioRxiv : the preprint server for biology·2024

Same author

Spatial Metabolome Lipidome and Glycome from a Single brain Section.

bioRxiv : the preprint server for biology·2023

Same journal

Editorial: Technologies for RNA Detection.

Bio-protocol·2026

Same journal

One-Step Affinity Purification of MarathonRT Reverse Transcriptase for RNA Sequencing Applications.

Bio-protocol·2026

Same journal

Enhanced RNA-Seq Expression Profiling and Functional Enrichment in Non-model Organisms Using Custom Annotations.

Bio-protocol·2026

Same journal

Using Combined Fluorescent In Situ Hybridization With Immunohistochemistry to Co-localize mRNA in Diverse Neuronal Cell Types.

Bio-protocol·2026

Same journal

Stepwise Protocol for Alternative Splicing Analysis in Single-Cell SMART-Seq2 RNA-Seq Data.

Bio-protocol·2026

Same journal

Enriching Bacteria-Specific RNA From Host Samples Before NGS With Transcript-Capture.

Bio-protocol·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Mar 17, 2026

Isolation of Region-specific Microglia from One Adult Mouse Brain Hemisphere for Deep Single-cell RNA Sequencing

Isolation of Region-specific Microglia from One Adult Mouse Brain Hemisphere for Deep Single-cell RNA Sequencing

Published on: December 3, 2019

Microsome Isolation from Tissue.

Maria Bodero¹, Jose Francisco Abisambra¹

¹Sanders-Brown Center on Aging and Department of Physiology, University of Kentucky, Lexington, USA.

|July 26, 2016

Summary

This summary is machine-generated.

This study details a refined protocol for extracting microsomes from frozen tissue. This method facilitates the examination of protein-protein interactions within the endoplasmic reticulum.

More Related Videos

Isolation of Cortical Microglia with Preserved Immunophenotype and Functionality From Murine Neonates

Isolation of Cortical Microglia with Preserved Immunophenotype and Functionality From Murine Neonates

Published on: January 30, 2014

Isolation and Culture of Rodent Microglia to Promote a Dynamic Ramified Morphology in Serum-free Medium

Isolation and Culture of Rodent Microglia to Promote a Dynamic Ramified Morphology in Serum-free Medium

Published on: March 9, 2018

Related Experiment Videos

Last Updated: Mar 17, 2026

Isolation of Region-specific Microglia from One Adult Mouse Brain Hemisphere for Deep Single-cell RNA Sequencing

Isolation of Region-specific Microglia from One Adult Mouse Brain Hemisphere for Deep Single-cell RNA Sequencing

Published on: December 3, 2019

Isolation of Cortical Microglia with Preserved Immunophenotype and Functionality From Murine Neonates

Isolation of Cortical Microglia with Preserved Immunophenotype and Functionality From Murine Neonates

Published on: January 30, 2014

Isolation and Culture of Rodent Microglia to Promote a Dynamic Ramified Morphology in Serum-free Medium

Isolation and Culture of Rodent Microglia to Promote a Dynamic Ramified Morphology in Serum-free Medium

Published on: March 9, 2018

Area of Science:

Biochemistry
Cell Biology
Molecular Biology

Background:

Microsomes are essential vesicles derived from the endoplasmic reticulum.
Understanding protein-protein interactions within the endoplasmic reticulum is crucial for cellular function.
Existing protocols may require optimization for specific downstream applications.

Purpose of the Study:

To present an optimized protocol for microsome extraction from frozen tissue.
To enable detailed examination of endoplasmic reticulum protein-protein interactions.

Main Methods:

Extraction of microsomes from frozen biological tissue.
Adaptation and modification of a previously published protocol (Abisambra et al., 2013).

Main Results:

Successful isolation of microsomes suitable for further analysis.
Optimized protocol enhances usability for subsequent protein interaction studies.

Conclusions:

The described protocol provides a reliable method for microsome isolation.
This optimized technique supports advanced research into endoplasmic reticulum protein dynamics.