Oxygen diffusion-concentration in erythrocyte plasma membranes studied by the fluorescence quenching of anionic and...

Fluorescence quenching by oxygen of cationic [pyrene-(CH2)nN(CH3)3+; n = 1, 4, and 11] and anionic [pyrene-(CH2)nCO2-, n = 3, 8, 11, and 15] probes was investigated in erythrocyte plasma membranes (leaky) in order to assess the ability of oxygen molecules to interact with solutes located at different positions in the membrane. The pseudounimolecular quenching rate constants measured increase, both for cationic and anionic probes, when n increases. These results are interpreted in terms of an increased oxygen solubility toward the center of the membrane interior, and imply that lateral diffusion contributes more than transverse diffusion to total oxygen mobility. For all of the probes considered, quenching rates increase when n-alkanols are added. The effect observed increases when n decreases and when the size of the n-alkanol alkyl chain increases. Arrhenius-type plots for the quenching rate constants show noticeable downward curvatures. Average (0-40 degrees C) activation energies are approximately 6 kcal/mol.