Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Chromatin aggregation changes substantially as pH varies within the physiological range.

X W Guo1, R D Cole

  • 1Department of Biochemistry, University of California, Berkeley 94720.

The Journal of Biological Chemistry
|July 15, 1989
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

[Structural equation analysis and modeling of upper limb WMSDs and their adverse ergonomic factors].

Zhonghua lao dong wei sheng zhi ye bing za zhi = Zhonghua laodong weisheng zhiyebing zazhi = Chinese journal of industrial hygiene and occupational diseases·2025
Same author

[Structural equation analysis and modeling of wrist WMSDs and its adverse ergonomic factors].

Zhonghua lao dong wei sheng zhi ye bing za zhi = Zhonghua laodong weisheng zhiyebing zazhi = Chinese journal of industrial hygiene and occupational diseases·2025
Same author

[Structural equation analysis and modeling of adverse ergonomic factors for lower back pain].

Zhonghua lao dong wei sheng zhi ye bing za zhi = Zhonghua laodong weisheng zhiyebing zazhi = Chinese journal of industrial hygiene and occupational diseases·2025
Same author

[Study on the relationship between occupational exposure characteristics and musculoskeletal disorders in 29 industries or workforce groups in China].

Zhonghua lao dong wei sheng zhi ye bing za zhi = Zhonghua laodong weisheng zhiyebing zazhi = Chinese journal of industrial hygiene and occupational diseases·2025
Same author

[Study on the epidemiological characteristics of work-related musculoskeletal disorders among 29 industries or occupational groups in China].

Zhonghua lao dong wei sheng zhi ye bing za zhi = Zhonghua laodong weisheng zhiyebing zazhi = Chinese journal of industrial hygiene and occupational diseases·2025
Same author

[Clinical and endoscopic characteristics of adult celiac disease].

Zhonghua nei ke za zhi·2023
Same journal

Isotope-Edited ESEEM: A New Method for Probing Copper Binding Sites in Neurodegenerative Proteins.

The Journal of biological chemistry·2026
Same journal

YhbO is a DJ-1 family glyoxalase and α-oxoaldehyde hydratase that confers resistance to reactive carbonyl stress (112).

The Journal of biological chemistry·2026
Same journal

ARMH3 acts as a central scaffold at the Golgi/TGN through interactions with Arl5, GBF1, and PI4KB.

The Journal of biological chemistry·2026
Same journal

PAX8 controls proximal tubule epithelial identity and stress response through epigenetic modification of distal regulatory elements.

The Journal of biological chemistry·2026
Same journal

Saturated cardiolipins are potent disruptors of inner mitochondrial membrane structure and function.

The Journal of biological chemistry·2026
Same journal

Phosphate release from myosin Va occurs after the initial powerstroke but before the secondary powerstroke associated with ADP-release.

The Journal of biological chemistry·2026
See all related articles

pH significantly influences chromatin aggregation and histone H1 dissociation in vitro. Increasing pH reduces chromatin aggregation, a key factor in cellular processes like mitosis.

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Background:

  • Chromatin aggregation is a critical process in cellular functions.
  • pH and salt concentrations are known environmental factors affecting biological molecules.

Purpose of the Study:

  • To investigate the in vitro effect of pH on chromatin aggregation.
  • To determine the relationship between pH, salt concentration, and chromatin structure.

Main Methods:

  • In vitro experiments were conducted to assess chromatin aggregation.
  • Varying pH and salt (NaCl, MgCl2) concentrations were used to observe their effects.

Main Results:

  • Chromatin aggregation was found to be highly dependent on pH and salt concentration.

Related Experiment Videos

  • Increasing pH led to decreased chromatin aggregation across all tested salt conditions.
  • Specific pH values (5.4, 6.0, 7.0, 8.3) were associated with half-maximal chromatin aggregation under different salt conditions.
  • A pH shift from 5.9 to 6.8 in 150 mM NaCl and 1 mM MgCl2 reduced aggregation from 86% to 63%, mirroring physiological pH changes during the cell cycle.
  • Histone H1 dissociation from chromatin was also observed to be pH-dependent.

    • Conclusions:

    • Physiological pH changes play a crucial role in regulating chromatin aggregation in vitro.
    • The findings suggest a mechanism by which pH influences chromatin structure and function during cellular processes like mitosis.
    • pH-dependent histone H1 dissociation may contribute to observed changes in chromatin organization.