The oligodendrocyte-specific antibody 'CC1' binds Quaking 7
Jenea M Bin1, Stephanie N Harris1, Timothy E Kennedy2
1Montreal Neurological Institute, Department of Neurology and Neurosurgery, McGill University, Montreal, Quebec, Canada.
Journal of Neurochemistry
|July 26, 2016
Summary
The CC1 antibody, commonly used to identify mature oligodendrocytes, actually binds Quaking 7 (QKI7). This RNA-binding protein is highly expressed in myelinating oligodendrocytes within the central nervous system.
Area of Science:
- Neuroscience
- Immunology
- Molecular Biology
Background:
- The anti-adenomatous polyposis coli (APC) clone CC1 antibody is widely used to label mature oligodendrocytes.
- Previous research indicated that CC1 binds an unknown antigen, despite being raised against APC.
Purpose of the Study:
- To identify the true antigen recognized by the CC1 antibody.
- To clarify the specificity of CC1 for oligodendrocyte labeling.
Main Methods:
- Immunohistochemistry
- Western blotting
- Antibody characterization
Main Results:
- The CC1 antibody specifically binds Quaking 7 (QKI7), an RNA-binding protein.
- QKI7 expression is significantly upregulated in myelinating oligodendrocytes in the central nervous system.
Conclusions:
- The CC1 antibody's target is QKI7, not APC.
- This finding refines the use of CC1 for studying myelinating oligodendrocytes.
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