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Published on: December 16, 2015
Development, Quantification, Method Validation, and Stability Study of a Novel Fucoxanthin-Fortified Milk
Il-Kyoon Mok1, Jung-Ro Yoon1, Cheol-Ho Pan
1Department of Food Processing and Distribution, Gangneung-Wonju National University , Gangneung, Gangwon-do 25457, Republic of Korea.
This study fortified milk with fucoxanthin, a marine carotenoid, and developed a reliable analysis method. Fucoxanthin stability in milk was assessed, revealing protein content influences its preservation, with skimmed milk offering better stability.
Area of Science:
- Marine biotechnology
- Food science
- Analytical chemistry
Background:
- Fucoxanthin, a marine carotenoid, has potential health benefits but limited food applications.
- Fortifying milk products offers a novel delivery system for fucoxanthin.
Purpose of the Study:
- To develop and validate a method for analyzing fucoxanthin in milk.
- To investigate the stability of fucoxanthin in whole milk (WM) and skimmed milk (SM) during processing and storage.
Main Methods:
- Milk fortification with fucoxanthin (8 μg/mL).
- Development and validation of a High-Performance Liquid Chromatography with Diode Array Detection (HPLC-DAD) method.
- Assessment of fucoxanthin stability during storage, pasteurization, and drying.
Main Results:
- A validated HPLC-DAD method with excellent linearity (R²=1.0000) was established.
- Fucoxanthin exhibited better stability in milk solutions over a 1-month storage period.
- Skimmed milk generally provided better fucoxanthin stability compared to whole milk, linked to protein content.
- Over 91% fucoxanthin retention was observed after three pasteurization cycles.
Conclusions:
- Milk is a viable food matrix for fucoxanthin fortification.
- Milk's protein content, particularly casein and whey protein, significantly impacts fucoxanthin stability.
- Skimmed milk demonstrates superior potential for preserving fucoxanthin compared to whole milk.
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