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Tris(hydroxymethyl)phosphine, P(CH2OH)3 - A convenient and effective new reagent for the fixation of protein samples
Megan J Wyllie1, Helen Turner2, William Henderson1
1School of Science, Faculty of Science and Engineering, University of Waikato, Private Bag 3105, Hamilton 3240, New Zealand.
Abstract:
Tris(hydroxymethyl)phosphine [P(CH2OH)3, THP] is demonstrated to be an alternative fixative to glutaraldehyde for preparation of proteinaceous specimens for SEM analysis. THP is easily prepared from commercially-available [P(CH2OH)4]Cl, and unlike many other alkylphosphines, is reasonably air-stable [>7days at pH 7-8]. Experiments carried out with chicken breast muscle, cross-sectioned perpendicular to the muscle fibres and imaged using SEM, indicate that THP effectively fixes the muscle structure with minimal discolouration of the sample.
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