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Updated: Mar 17, 2026
Labeling Stem Cells with Ferumoxytol, an FDA-Approved Iron Oxide Nanoparticle
Published on: November 4, 2011
Stem cell labeling with iron oxide nanoparticles: impact of 3D culture on cell labeling maintenance
Teresa Kilian1, Florian Fidler2, Annika Kasten3
1Fraunhofer Institute for Interfacial Engineering & Biotechnology IGB, Translational Center "Regenerative Therapies for Oncology & Musculoskeletal Diseases" - Würzburg branch, Röntgenring 11, 97070 Würzburg, Germany.
Aim:
We aimed to analyze the suitability of nanoparticles (M4E) for safe human mesenchymal stem cell (hMSC) labeling and determined cell labeling maintenance in 2D and 3D culture.
Materials & Methods:
We investigated cell-particle interaction and the particles' impact on cell viability, growth and proliferation. We analyzed cell labeling maintenance in 2D and 3D culture invasively and noninvasively.
Results:
M4E do not affect cell viability, growth and proliferation and do not cause chromosomal aberrations. Cell labeling maintenance is up to five-times higher in 3D conditions compared with 2D culture.
Conclusion:
M4E allow safe hMSC labeling and noninvasive identification. Our hMSC-loaded, 3D tissue-engineered construct could serve as a graft for regenerative therapies, in which M4E-labeled hMSCs can migrate to their target.
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