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Transient Expression in Nicotiana Benthamiana Leaves for Triterpene Production at a Preparative Scale
Published on: August 16, 2018
Triterpenoids from the branch and leaf of Abies fargesii
Wenming Wu1, Xu Chen1, Ye Liu1
1Faculty of Pharmacy, Tongji Medical College of Huazhong University of Science and Technology, Hangkonglu 13, Wuhan 430030, People's Republic of China.
Abstract:
Eighteen triterpenoids, abifarines A-R, including fourteen previously unknown and four artifacts, together with six known ones, were isolated from the branch and leaf of Abies fargesii. Their structures were elucidated by spectroscopic data analysis. The relative configurations of abifarines A and F were further confirmed by single-crystal X-ray diffraction analysis with Mo Kα irradiation. All compounds were evaluated for their in vitro cytotoxicity against the mouse cancer B16 cell line, and human cancer HepG2 and MCF7 cell lines. (24R)-cycloartane-3β,24,25-triol and (24R)-cycloartane-3β,24,25,28-tetrol showed moderate anti-proliferative effect for B16 and HepG2 cell lines with IC50 values of 32.9, 19.5 μM and 26.4, 21.5 μM, respectively.
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