Related Experiment Video
Updated: Mar 17, 2026
Facial Nerve Surgery in the Rat Model to Study Axonal Inhibition and Regeneration
Published on: May 5, 2020
An automated whisker tracking tool for the rat facial nerve injury paradigm
Benjamin Dorschner1, Herbert Süße2, Wolfgang Ortmann2
1Department of Otorhinolaryngology, Jena University Hospital, Lessingstrasse 2, D-07743 Jena, Germany; Department of Pediatrics, University Hospital Carl Gustav Carus, Fetscherstrasse 74, D-01307 Dresden, Germany.
Background:
The two-dimensional videographic analysis of vibrissal movements in behaving rodents has become a standard method to estimate the degree of functional impairment and recovery after facial nerve injuries quantitatively. The main limitation of the method is the time consuming, uneconomic process of manually tracking the vibrissae in video sequences.
New Method:
We developed a novel tool allowing automated detection of untagged vibrissae (two on each side of the snout). To compare the new method with the standard manual tracking approach, we used videos of unrestrained rats with unilateral section and immediate suture of the facial nerve performed two months earlier.
Results:
Measurement agreement analyses showed that the two methods are equivalent for both "normal" high-amplitude vibrissal movements (non-operated side) and low-amplitude whisking (reinnervated side). Spectral analysis revealed a significant deviation in the power spectra on the control and injured side, indicating that bilaterally coordinated whisker movements are not present two months after surgery.
Comparison With Existing Method(S):
The novel method yields results equal to those of the manual tracking approach. An advantage of our tool is the possibility to significantly increase sample size without additional labor cost.
Conclusions:
The novel tool can increase the efficacy and spectrum of functional measures used in facial nerve regeneration research.
