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Updated: Mar 17, 2026

Author Spotlight: Quantification of Complex Lipidomic Samples Using Stable Isotope Labeling
Published on: August 23, 2024
Structural identification of triacylglycerol isomers using electron impact excitation of ions from organics (EIEIO)
Takashi Baba1, J Larry Campbell2, J C Yves Le Blanc2
1SCIEX, Concord, Ontario, Canada takashi.baba@sciex.com.
Abstract:
Electron-induced dissociation or electron impact excitation of ions from organics (EIEIO) was applied to triacylglycerols (TAGs) for in-depth molecular structure analysis using MS. In EIEIO, energetic electrons (∼10 eV) fragmented TAG ions to allow for regioisomeric assignment of identified acyl groups at the sn-2 or sn-1/3 positions of the glycerol backbone. In addition, carbon-carbon double bond locations within the acyl chains could also be assigned by EIEIO. Beyond the analysis of lipid standards, this technique was applied to edible oils and natural lipid extracts to demonstrate the power of this method to provide in-depth structural elucidation of TAG molecular species.
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