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Immunogold Electron Microscopy

Immunogold Electron Microscopy

Immunoelectron microscopy utilizes immunogold labeling of endogenous proteins with specific antibodies to detect and localize these proteins in cells and tissues. The procedure provides insights into the distribution and quantification of protein under different stimulation conditions offering clues about their functions. Conjugating highly electron-dense gold particles with primary or secondary antibodies allow antigen detection on and within cells, with high resolution and specificity.

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Related Experiment Video

Updated: Mar 17, 2026

Synthesis, Characterization, and Application of Superparamagnetic Iron Oxide Nanoprobes for Extrapulmonary Tuberculosis Detection

Synthesis, Characterization, and Application of Superparamagnetic Iron Oxide Nanoprobes for Extrapulmonary Tuberculosis Detection

Published on: February 16, 2020

Interactions Between Antibody-Coated Iron Micro-Particles and Leukemic Cells as seen by Electron Microscopy.

C J Bieva¹, R Mosselmans², F V Brugghen¹

¹a Dept. Hématologie, Service de Médicine Interne et Laboratoire d'Investigation Clinique H. Tagnon, Brussels, Belgium.

Leukemia & Lymphoma

|July 27, 2016

Summary

Monoclonal antibody-coated microparticles target leukemic cells. CD10 antibody-coated particles penetrate cells at 37°C, offering potential for targeted drug delivery in cancer therapy.

Keywords:

Antibody-coated particles electron microscopy iron particles leukemic cells

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Last Updated: Mar 17, 2026

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Published on: February 16, 2020

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Using Magnetometry to Monitor Cellular Incorporation and Subsequent Biodegradation of Chemically Synthetized Iron Oxide Nanoparticles

Using Magnetometry to Monitor Cellular Incorporation and Subsequent Biodegradation of Chemically Synthetized Iron Oxide Nanoparticles

Published on: February 27, 2021

Area of Science:

Biomedical Engineering
Cell Biology
Immunology

Background:

Monoclonal antibodies (MoAbs) conjugated to ferromagnetic microparticles are used for purging leukemic cells from autografts.
Previous studies have reported the properties of this reagent for autologous bone marrow transplantation (ABMT).

Purpose of the Study:

To visualize the interaction between MoAb-coated ferromagnetic microparticles and leukemic cells using electron microscopy.
To investigate the effect of temperature and incubation time on particle-cell interactions.

Main Methods:

Transmission electron microscopy was employed to observe interactions.
A CD10+ NALM6- cell suspension was incubated with CD10 or CD8 MoAb-conjugated particles.
Incubation occurred over a time course (0-90 min) at 4°C and 37°C.

Main Results:

CD10 MoAb-particles complexed with the cell surface at 4°C.
At 37°C, CD10 MoAb-particles readily penetrated leukemic cells.
CD8 MoAb-particles showed no interaction with cells at either temperature or duration.

Conclusions:

Temperature-dependent cellular uptake of CD10 MoAb-conjugated microparticles was demonstrated.
This suggests a potential for site-specific drug delivery by internalizing pharmaceutical agents linked to magnetic carriers.
The CD8 MoAb-particle interaction was negligible, highlighting the specificity of the CD10-mediated uptake.