Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Flow Cytometry01:23

Flow Cytometry

17.1K
The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
In...
17.1K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Cellular image analysis and imaging by flow cytometry.

Clinics in laboratory medicine·2007
Same author

Extended depth of field imaging for high speed cell analysis.

Cytometry. Part A : the journal of the International Society for Analytical Cytology·2007
Same author

Sensitivity measurement and compensation in spectral imaging.

Cytometry. Part A : the journal of the International Society for Analytical Cytology·2006
Same author

Distinguishing modes of cell death using the ImageStream multispectral imaging flow cytometer.

Cytometry. Part A : the journal of the International Society for Analytical Cytology·2004

Related Experiment Video

Updated: Mar 17, 2026

Qualitative and Quantitative Analysis of the Immune Synapse in the Human System Using Imaging Flow Cytometry
08:35

Qualitative and Quantitative Analysis of the Immune Synapse in the Human System Using Imaging Flow Cytometry

Published on: January 7, 2019

12.3K

Principles of Amnis Imaging Flow Cytometry.

David A Basiji1

  • 1Department of Bioengineering, University of Washington, Seattle, WA, USA. dbasiji@comcast.net.

Methods in Molecular Biology (Clifton, N.J.)
|July 28, 2016
PubMed
Summary

Imaging flow cytometry (IFC) is a rapidly advancing technique, now used in over 500 studies. Despite its growth, IFC remains an emerging technology with significant potential for further development and adoption in scientific research.

Keywords:
Flow cytometryImagingTime delay integration

More Related Videos

Automation of the Micronucleus Assay Using Imaging Flow Cytometry and Artificial Intelligence
09:11

Automation of the Micronucleus Assay Using Imaging Flow Cytometry and Artificial Intelligence

Published on: January 27, 2023

3.0K
Flow Cytometric Analysis of Bimolecular Fluorescence Complementation: A High Throughput Quantitative Method to Study Protein-protein Interaction
11:11

Flow Cytometric Analysis of Bimolecular Fluorescence Complementation: A High Throughput Quantitative Method to Study Protein-protein Interaction

Published on: August 15, 2013

19.0K

Related Experiment Videos

Last Updated: Mar 17, 2026

Qualitative and Quantitative Analysis of the Immune Synapse in the Human System Using Imaging Flow Cytometry
08:35

Qualitative and Quantitative Analysis of the Immune Synapse in the Human System Using Imaging Flow Cytometry

Published on: January 7, 2019

12.3K
Automation of the Micronucleus Assay Using Imaging Flow Cytometry and Artificial Intelligence
09:11

Automation of the Micronucleus Assay Using Imaging Flow Cytometry and Artificial Intelligence

Published on: January 27, 2023

3.0K
Flow Cytometric Analysis of Bimolecular Fluorescence Complementation: A High Throughput Quantitative Method to Study Protein-protein Interaction
11:11

Flow Cytometric Analysis of Bimolecular Fluorescence Complementation: A High Throughput Quantitative Method to Study Protein-protein Interaction

Published on: August 15, 2013

19.0K

Area of Science:

  • Biotechnology
  • Cell Biology
  • Analytical Chemistry

Background:

  • Imaging flow cytometry (IFC) has gained prominence over the past decade, appearing in over 500 scientific publications.
  • Despite its increasing adoption, IFC represents a small fraction of the installed base compared to conventional flow cytometry.
  • The technology has undergone rapid evolution, enhancing speed, sensitivity, and data analysis capabilities.

Purpose of the Study:

  • To summarize the fundamental principles of operation for imaging flow cytometry.
  • To present the current state-of-the-art in IFC technology.

Main Methods:

  • Review of imaging flow cytometry principles.
  • Analysis of technological advancements and current capabilities.

Main Results:

  • IFC is an established, yet emerging, technique with substantial growth in scientific literature.
  • Significant improvements in speed, sensitivity, and real-time data analysis have been achieved.

Conclusions:

  • Imaging flow cytometry is a powerful and evolving tool in biological and chemical analysis.
  • The continuous technological advancements position IFC for broader application and impact in research.