Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Flow Cytometry01:23

Flow Cytometry

17.1K
The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
In...
17.1K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Erratum for Levesque et al., "Complete genome sequence of bacteriophages Merry and Sunny infecting <i>Microbacterium chocolatum</i> strain GAI20246-6 isolated from an outdoor commercial algal pond".

Microbiology resource announcements·2026
Same author

Complete genome sequence of bacteriophages Merry and Sunny infecting <i>Microbacterium chocolatum</i> strain <i>GAI20246-6</i> isolated from an outdoor commercial algal pond.

Microbiology resource announcements·2025
Same author

<i>Rhizosolenia</i> mat diatoms associate with nitrogen-fixing microbes.

ISME communications·2025
Same author

Immune Response of Nile Tilapia (Oreochromis niloticus) Vaccinated With Diatom-Based Oral Vaccines Against Piscine Francisellosis.

Journal of fish diseases·2025
Same author

Size and fluorescence calibrated imaging flow cytometry: From arbitrary to standard units.

Cytometry. Part A : the journal of the International Society for Analytical Cytology·2024
Same author

The V-type ATPase enhances photosynthesis in marine phytoplankton and further links phagocytosis to symbiogenesis.

Current biology : CB·2023

Related Experiment Video

Updated: Mar 17, 2026

Microfluidic Imaging Flow Cytometry by Asymmetric-detection Time-stretch Optical Microscopy ATOM
07:19

Microfluidic Imaging Flow Cytometry by Asymmetric-detection Time-stretch Optical Microscopy ATOM

Published on: June 28, 2017

10.8K

Applications of Imaging Flow Cytometry for Microalgae.

Mark Hildebrand1, Aubrey Davis2, Raffaela Abbriano2

  • 1Marine Biology Research Division, Scripps Institution of Oceanography, University of California, San Diego, La Jolla, CA, USA. mhildebrand@ucsd.edu.

Methods in Molecular Biology (Clifton, N.J.)
|July 28, 2016
PubMed
Summary

Imaging flow cytometry enhances the analysis of microalgae by enabling high-resolution imaging of intracellular features. This advanced technique aids in understanding cellular responses and gene expression in diverse microalgal populations.

Keywords:
Imaging cytometryIntracellular processesMicroalgaePhytoplanktonPopulation heterogeneity

More Related Videos

Author Spotlight: Advancing Research in Microbial Autoaggregation Using Imaging Flow Cytometry
05:19

Author Spotlight: Advancing Research in Microbial Autoaggregation Using Imaging Flow Cytometry

Published on: September 29, 2023

1.4K
Characterization of Aquatic Biofilms with Flow Cytometry
08:30

Characterization of Aquatic Biofilms with Flow Cytometry

Published on: June 6, 2018

9.7K

Related Experiment Videos

Last Updated: Mar 17, 2026

Microfluidic Imaging Flow Cytometry by Asymmetric-detection Time-stretch Optical Microscopy ATOM
07:19

Microfluidic Imaging Flow Cytometry by Asymmetric-detection Time-stretch Optical Microscopy ATOM

Published on: June 28, 2017

10.8K
Author Spotlight: Advancing Research in Microbial Autoaggregation Using Imaging Flow Cytometry
05:19

Author Spotlight: Advancing Research in Microbial Autoaggregation Using Imaging Flow Cytometry

Published on: September 29, 2023

1.4K
Characterization of Aquatic Biofilms with Flow Cytometry
08:30

Characterization of Aquatic Biofilms with Flow Cytometry

Published on: June 6, 2018

9.7K

Area of Science:

  • Microbiology
  • Cell Biology
  • Biotechnology

Background:

  • Flow cytometry is a powerful tool for analyzing cells and cell populations.
  • Imaging capabilities significantly enhance flow cytometry by allowing visualization of intracellular features.
  • Unicellular microalgae present opportunities for high-throughput analysis due to their diversity and varied cellular responses.

Purpose of the Study:

  • To review applications of imaging cytometry for characterizing photosynthetic microalgae.
  • To demonstrate the advantages of imaging flow cytometry for specific research objectives.
  • To illustrate the value of cytometric evaluation for microalgae analysis, including population studies, feature documentation, and gene expression analysis.

Main Methods:

  • Utilized Amnis® ImageStream®X instrument for imaging cytometry.
  • Applied high-throughput analysis to unicellular microalgae.
  • Compared imaging flow cytometry with conventional flow cytometry for specific analyses.

Main Results:

  • Demonstrated the ability to image large numbers of cells at high resolution.
  • Showcased the correlation between molecular phenomena and cellular phenotype alterations.
  • Highlighted the utility of imaging flow cytometry for analyzing populations, documenting cellular features, and assessing gene expression in microalgae.

Conclusions:

  • Imaging flow cytometry offers unique advantages for detailed cellular analysis, particularly for microalgae.
  • The technique enables comprehensive characterization of microalgal diversity and responses.
  • Imaging cytometry is valuable for population analysis, feature documentation, and gene expression studies in microalgae.