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Screening for Drugs Against the Plasmodium falciparum Digestive Vacuole by Imaging Flow Cytometry
Yan Quan Lee1,2, Brian E Hall3, Kevin S W Tan4,5
1Department of Microbiology, National University of Singapore, Blk MD4, Level 3, 5 Science Drive 2, Singapore, 117545, Singapore.
Methods in Molecular Biology (Clifton, N.J.)
|July 28, 2016
Abstract:
Phenotypic assays are increasingly employed to provide clues about drug mechanisms. In antimalarial drug screening, however, the majority of assays are designed to only measure parasite-killing activity. We describe here a high-content assay to detect drug-mediated perturbation of the digestive vacuole integrity in the trophozoite stage of Plasmodium falciparum, using the ImageStream imaging flow cytometer.
Keywords:
ApoptosisDigestive vacuoleDrug discoveryFluo-4Imaging flow cytometryMalariaPlasmodium falciparumProgrammed cell death
