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A High-throughput Platform for the Screening of Salmonella spp./Shigella spp.
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A comparison of flocked swabs and traditional swabs, using multiplex real-time PCR for detection of common
Margaret Mokomane1, Ishmael Kasvosve2, Simani Gaseitsiwe3
1Department of Medical Laboratory Sciences, Faculty of Health Sciences University of Botswana, Gaborone, Botswana; Botswana National Health Laboratory, Gaborone, Botswana.
Diagnostic Microbiology and Infectious Disease
|July 28, 2016
Abstract:
We compared the performance of flocked and matched traditional rectal swabs collected from 236 children admitted with gastroenteritis in Botswana. All samples were tested using real time multiplex-PCR assays for nine enteric pathogens. There was a 20% higher detection of Shigella from flocked swabs, but most other pathogens had similar detection rates.

