Jove
Visualize
Contact Us

Related Experiment Videos

A practicable two-dimensional electrophoretic method for routine analysis of urinary proteins.

A Lapin1

  • 1Institut für Klinische Chemie und Laboratoriumsdiagnostik, Universität Wien.

Journal of Clinical Chemistry and Clinical Biochemistry. Zeitschrift Fur Klinische Chemie Und Klinische Biochemie
|February 1, 1989
PubMed
Summary

This study introduces a rapid, reliable two-dimensional electrophoresis method for analyzing urinary proteins. The technique enhances the identification of specific protein markers for various pathological conditions.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Increased soluble transferrin receptor in a case of hypernephroma accompanied by polycythaemia and elevated erythropoietin.

Wiener medizinische Wochenschrift (1946)·2002
Same author

Antibodies against oxidized LDL--theory and clinical use.

Physiological research·2001
Same author

Mediating mechanisms in a program to reduce intentions to use anabolic steroids and improve exercise self-efficacy and dietary behavior.

Prevention science : the official journal of the Society for Prevention Research·2001
Same author

Feto-maternal hemorrhage after a car accident: a case report.

Acta obstetricia et gynecologica Scandinavica·2001
Same author

Autoantibodies against oxidized LDL in the first phase of life. Low density lipoproteins.

Clinical chemistry and laboratory medicine·1999
Same author

[Laboratory findings in elderly patients: a forgotten aspect of laboratory medicine?].

Zeitschrift fur Gerontologie und Geriatrie·1999
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Area of Science:

  • Clinical Chemistry
  • Proteomics
  • Biochemical Analysis

Background:

  • Accurate analysis of urinary proteins is crucial for diagnosing and monitoring various kidney diseases and systemic conditions.
  • Existing one-dimensional electrophoresis methods can be limited in their ability to resolve complex protein mixtures and identify specific disease markers.
  • There is a need for robust, efficient, and reproducible methods for routine clinical analysis of urinary protein profiles.

Purpose of the Study:

  • To describe and validate a novel two-dimensional electrophoresis (2DE) method for the routine clinical analysis of urinary proteins.
  • To demonstrate the enhanced ability of this 2DE method to identify specific protein markers and characteristic changes in protein patterns compared to traditional methods.

Main Methods:

Related Experiment Videos

  • A two-dimensional electrophoresis technique combining cellulose acetate electrophoresis (first dimension) and sodium dodecyl sulfate (SDS) electrophoresis (second dimension).
  • Utilized the 'Phast System' for SDS electrophoresis and gel staining with Coomassie Blue, employing ready-to-use separation media.
  • The entire analytical process was completed within 100 minutes, emphasizing efficiency and reproducibility.

    • Main Results:

    • The developed 2DE method provides a reliable and reproducible analysis of urinary proteins.
    • Visual evaluation of the two-dimensional protein pattern, considering molecular weight distribution and the five-zone cellulose acetate pattern, allows for clear interpretation.
    • Specific protein markers and characteristic alterations in protein spot constellations, indicative of pathological changes, were more readily identified than with one-dimensional SDS electrophoresis.

    Conclusions:

    • The described 2DE method is suitable for routine clinical analysis of urinary proteins, offering improved diagnostic insights.
    • This technique facilitates easier recognition and evaluation of specific protein markers associated with pathological conditions.
    • The method's efficiency, reliability, and enhanced discriminatory power make it a valuable tool in clinical proteomics and diagnostics.