Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Master Transcription Regulators02:23

Master Transcription Regulators

8.0K
Master transcription regulators are regulatory proteins that are predominantly responsible for regulating the expression of multiple genes. Often these genes work in concert to drive a  complex process. Activation of a master transcription regulator can lead to a cascade of transcriptional activation necessary for that outcome. These regulators can directly bind to the regulatory sequences of the various genes involved, or they can indirectly regulate transcription by binding to regulatory...
8.0K
Histone Variants at the Centromere02:30

Histone Variants at the Centromere

5.2K
Histone variants are the histone proteins with structural and sequence variations. These variants may be regarded as “mutant” forms that replace their canonical histone counterparts in the nucleosomes. Specific post-translational modifications on the histone variants enable further chromatin complexity and regulate tissue-specific gene expression. The most common histone variants are from histone H2A, H2B, and linker histone H1 families. However, several variants of histone H3...
5.2K
Cooperative Binding of Transcription Regulators02:13

Cooperative Binding of Transcription Regulators

7.5K
Transcriptional regulators bind to specific cis-regulatory sequences in the DNA to regulate gene transcription. These cis-regulatory sequences are very short, usually less than ten nucleotide pairs in length. The short length means that there is a high probability of the exact same sequence randomly occurring throughout the genome.  Since regulators can also bind to groups of similar sequences, this further increases the chances of random binding. Transcriptional regulators form...
7.5K
Heterochromatin02:38

Heterochromatin

18.9K
The extent of chromatin compaction can be studied by staining chromatin using specific DNA binding dyes. Under the microscope, the dense-compacted regions that take up more dye are called heterochromatin. Heterochromatin is further classified into two forms – constitutive heterochromatin and facultative heterochromatin.
Constitutive heterochromatin: It is a highly compact region of chromatin that is mostly concentrated in the centromere and telomere. Unlike euchromatin, the amino acid at...
18.9K
Single-Strand DNA Binding Proteins01:03

Single-Strand DNA Binding Proteins

17.1K
For successful DNA replication, the unwinding of double-stranded DNA must be accompanied by stabilization and protection of the separated single strands of the DNA. This crucial task is performed by single-strand DNA-binding (SSB) proteins. They bind to the DNA in a sequence-independent manner, which means that the nitrogenous bases of the DNA need not be present in a specific order for binding of SSB proteins to it. The binding of SSB proteins straightens single-stranded DNA (ssDNA) and makes...
17.1K
Spreading of Chromatin Modifications02:25

Spreading of Chromatin Modifications

9.9K
The histone proteins in the nucleosomes are post-translationally modified (PTM) to increase or decrease access to DNA. The commonly observed PTMs are methylation, acetylation, phosphorylation, and ubiquitination of lysine amino acids in the histone H3 tail region. These histone modifications have specific meaning for the cell. Hence, they are called "histone code". The protein complex involved in histone modification is termed as "reader-writer" complex.
Writers
The writer...
9.9K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Dysfunction of a SET3-like complex underlies a family of related neurological disorders.

Nature communications·2026
Same author

MeCP2 requires interactions with nucleosome linker DNA to read chromatin DNA methylation.

Nature communications·2026
Same author

Modelling transcription with explainable AI uncovers context-specific epigenetic gene regulation at promoters and gene bodies.

PLoS genetics·2025
Same author

A Regulatory Perspective on a UK Federated Data Network for Medicines and Medical Devices: Lessons from a 'Study-A-Thon'.

Therapeutic innovation & regulatory science·2025
Same author

Cohesin as an essential disruptor of chromosome organization.

Molecular cell·2025
Same author

Time Series Methods to Assess the Impact of Regulatory Action: A Study of UK Primary Care and Hospital Data on the Use of Fluoroquinolones.

Pharmacoepidemiology and drug safety·2024

Related Experiment Video

Updated: Mar 17, 2026

An Electrochemiluminescence-Based Assay for MeCP2 Protein Variants
07:44

An Electrochemiluminescence-Based Assay for MeCP2 Protein Variants

Published on: May 22, 2020

28.5K

Sequence-specific DNA binding by AT-hook motifs in MeCP2.

Matthew J Lyst1, John Connelly1, Cara Merusi1

  • 1The Wellcome Trust Centre for Cell Biology, University of Edinburgh, Edinburgh, UK.

FEBS Letters
|July 28, 2016
PubMed
Summary

Methyl-CpG-binding protein 2 (MeCP2) has two AT-hook motifs. These motifs are secondary contributors to MeCP2

Area of Science:

  • Molecular biology
  • Genetics
  • Biochemistry

Background:

  • MeCP2 is a chromatin protein implicated in Rett syndrome.
  • MeCP2 binds methylated DNA via its methyl-CpG-binding domain.
  • Alternative recruitment mechanisms for MeCP2 to the genome have been proposed.

Discussion:

  • This study investigated the DNA binding specificity of two AT-hook motifs in MeCP2 using in vitro and in vivo assays.
  • One AT-hook motif demonstrated strong sequence-specific DNA binding, while the other showed weaker binding.
  • These findings suggest the AT-hook motifs play a secondary role in MeCP2's overall DNA binding.

Key Insights:

  • The AT-hook motifs in MeCP2 contribute secondarily to its DNA binding.
  • Sequence-specific DNA binding was observed for one AT-hook motif.
Keywords:
AT-hookMeCP2Rett syndrome

More Related Videos

Pooled shRNA Screen for Reactivation of MeCP2 on the Inactive X Chromosome
11:15

Pooled shRNA Screen for Reactivation of MeCP2 on the Inactive X Chromosome

Published on: March 2, 2018

7.7K
CD Spectroscopy to Study DNA-Protein Interactions
06:48

CD Spectroscopy to Study DNA-Protein Interactions

Published on: February 10, 2022

7.8K

Related Experiment Videos

Last Updated: Mar 17, 2026

An Electrochemiluminescence-Based Assay for MeCP2 Protein Variants
07:44

An Electrochemiluminescence-Based Assay for MeCP2 Protein Variants

Published on: May 22, 2020

28.5K
Pooled shRNA Screen for Reactivation of MeCP2 on the Inactive X Chromosome
11:15

Pooled shRNA Screen for Reactivation of MeCP2 on the Inactive X Chromosome

Published on: March 2, 2018

7.7K
CD Spectroscopy to Study DNA-Protein Interactions
06:48

CD Spectroscopy to Study DNA-Protein Interactions

Published on: February 10, 2022

7.8K
  • The lack of disease-associated mutations in these motifs supports their non-critical role in DNA binding.

    • Outlook:

    • Further research could explore the interplay between the AT-hook motifs and the methyl-CpG-binding domain.
    • Understanding MeCP2's DNA binding is crucial for developing therapies for Rett syndrome.
    • Investigating other potential DNA-binding domains or interactions of MeCP2 may reveal additional functions.