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BRAF-activated LncRNA functions as a tumor suppressor in papillary thyroid cancer

Tian Liao1, Ning Qu1, Rong-Liang Shi1,2

  • 1Department of Head and Neck Surgery, Fudan University Shanghai Cancer Center, Department of Oncology, Shanghai Medical College, Fudan University, Shanghai, 200032, China.

Oncotarget
|July 28, 2016
PubMed

Insights

BRAF-activated non-coding RNA (BANCR) acts as a tumor suppressor in papillary thyroid carcinoma (PTC). Lower BANCR levels correlate with advanced PTC, and its overexpression inhibits tumor growth and metastasis.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Long non-coding RNAs (lncRNAs) are implicated in various cancer hallmarks.
  • BRAF-activated non-coding RNA (BANCR) exhibits dual roles as an oncogene and tumor suppressor.
  • The specific function of BANCR in papillary thyroid carcinoma (PTC) requires elucidation.

Purpose of the Study:

  • To investigate the role of BANCR in papillary thyroid carcinoma (PTC).
  • To assess BANCR expression levels in PTC tissues and cell lines.
  • To determine the impact of BANCR modulation on PTC cell behavior and tumor growth.

Main Methods:

  • Quantitative real-time PCR (qRT-PCR) to measure BANCR levels in 92 patient tissues.
  • Lentiviral vectors used to overexpress BANCR in PTC cell lines.
  • In vitro assays for proliferation, apoptosis, migration, and invasion.
  • Xenograft mouse models to evaluate tumor growth inhibition.

Main Results:

  • BANCR expression was significantly lower in PTC tissues compared to normal tissues.
  • Reduced BANCR levels correlated with larger tumor size, multifocal lesions, and advanced stage.
  • BANCR overexpression suppressed PTC cell proliferation, induced apoptosis, and inhibited migration/invasion.
  • Overexpression of BANCR inactivated ERK1/2 and p38 signaling pathways.
  • BANCR overexpression significantly inhibited tumor growth in vivo.

Conclusions:

  • BANCR functions as a tumor suppressor in papillary thyroid carcinoma.
  • Decreased BANCR expression is associated with PTC progression and poor prognostic indicators.
  • BANCR holds potential as a novel prognostic biomarker for PTC.
Keywords:
BRAFlncRNAmigrationpapillary thyroid cancerproliferation

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