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Published on: June 9, 2023
Surface modification of SOI-FET sensors for label-free and specific detection of short RNA analyte
Elena Dmitrienko1, Olga Naumova2, Boris Fomin2
1SB RAS, Institute of Chemical Biology & Fundamental Medicine, 8 Lavrentiev Avenue, Novosibirsk 630090, Russia.
Aim:
A new type of surface modification of SOI-FET sensors with ultrathin sensor-probe transition layer and uncharged probes for highly specific detection of short RNA (saRNA) was suggested.
Materials & Methods:
Carbonyldiimidazole (CDI) or glycidoxypropyltrimethoxysilane were used as precursors of sensor-probe interface layers, together with peptide nucleic acids and new NA analogues - phosphoryl guanidine oligo(2'-OMe)ribonucleotides (PGO) as probes for RNA hybridization. RNA sequences corresponding to mRNA NELFA (NM_005663) and microRNA-29a (cancer markers) were used as saRNA targets. Real-time saRNA detection by SOI-FET sensors and fluorescence analysis were applied.
Results:
Highly specific response with femtomolar sensitivity to saRNA was demonstrated for CDI-PGO-modified sensors fabricated by optical lithography.
Conclusion:
The proposed CDI-PGO protocol of modification of Si sensor surface is a promising procedure for biomedical applications.
