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Related Experiment Video

Updated: Mar 17, 2026

A Syngeneic Mouse B-Cell Lymphoma Model for Pre-Clinical Evaluation of CD19 CAR T Cells

A Syngeneic Mouse B-Cell Lymphoma Model for Pre-Clinical Evaluation of CD19 CAR T Cells

Published on: October 16, 2018

CD5(+) B Cell Lymphomas of Mice.

G Haughton¹, L W Arnold¹

¹a Department of Microbiology and Immunology, University of North Carolina at Chapel Hill, Chapel Hill, NC, 27599, USA.

Leukemia & Lymphoma

|July 28, 2016

Summary

Murine lymphomas originate from CD5 B cells, with early neoplastic commitment preceding late-life manifestation. This process involves skewed immunoglobulin V gene usage, similar to normal CD5 B cells, suggesting antigen-driven selection in early life.

Area of Science:

Immunology
Oncology
Genetics

Background:

Spontaneous murine lymphomas frequently originate from CD5 B cells, appearing late in life.
Lymphoma development involves an early commitment to neoplasia followed by a long indolent phase.
The immunoglobulin V gene repertoire in these lymphomas is not random, showing specific VH and VK gene associations.

Purpose of the Study:

To investigate the origins and developmental processes of spontaneous murine lymphomas.
To understand the role of immunoglobulin gene repertoire skewing in lymphomagenesis.
To characterize the specific V gene usage and antibody specificities associated with CD5 B cell lymphomas.

Main Methods:

Analysis of immunoglobulin (Ig) V gene repertoire in spontaneous murine lymphomas.

Keywords:

CD5 + B-cell lymphoma murine lymphoma

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Comparison of lymphoma Ig V gene usage with that of normal CD5 B cells.

Investigation of CD5 B cell development and repertoire selection in mice.

Main Results:

The unbalanced Ig V gene repertoire in lymphomas mirrors that of normal CD5 B cells, indicating lymphomagenesis occurs after repertoire establishment.
CD5 B cell repertoire skewing happens early in post-natal life, driven by antigen selection, primarily self-antigens.
A subset of CD5 B cells and lymphomas express VH11/VH12 genes with specific VK genes, producing anti-phosphatidylcholine antibodies, a specificity not found in conventional B cells.

Conclusions:

Murine CD5 B cell lymphomas arise from a pre-established, skewed V gene repertoire.
Antigen-driven selection by self-antigens during early post-natal development shapes the CD5 B cell repertoire and predisposes to lymphomagenesis.
Specific VH gene usage and antibody specificities characterize these lymphomas, highlighting a distinct B cell subset involved in lymphomagenesis.