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Updated: Mar 17, 2026
Bacterial Detection & Identification Using Electrochemical Sensors
Published on: April 23, 2013
Electrochemical sensing of biomarker for diagnostics of bacteria-specific infections
Fatima AlZahra'a Alatraktchi1,2,3, Helle Krogh Johansen3,4, Søren Molin2,3
1Department of Micro- & Nanotechnology, Technical University of Denmark, Kgs-Lyngby, Denmark.
Aim:
Pseudomonas aeruginosa is a pathogen that is prevalent in serious infections in compromised patients worldwide. A unique virulence factor of this bacterium is the redox-active molecule pyocyanin, which is a potential biomarker for the identification of P. aeruginosa infections. Here we report a direct, selective and rapid detection technique of pyocyanin.
Materials & Methods:
Pyocyanin was detected by amperometry at a relatively high potential where the pyocyanin signal was unaffected by background contributions.
Results & Conclusion:
Pyocyanin was detected at concentrations down to 125 nM in a 50 µM mixture of interfering compounds with a reproducibility of r(2) = 0.999 (n = 5) within 200 s. The results document a step toward a point-of-care technique for diagnosis of P. aeruginosa infections.
