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A Simple Red Blood Cell Lysis Method for the Establishment of B Lymphoblastoid Cell Lines

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Early Pre-B Lymphoblastic Transformation in A Patient with Refractory Anemia.

M Rozman¹, J M Ribera¹, A U Ispizua¹

¹a Postgraduate School of Hematology "Farreras Valenti". Hospital Clinic. University of Barcelona, Spain.

Leukemia & Lymphoma

|July 28, 2016

Summary

This study reports a rare case of acute lymphoblastic leukemia transformation in a patient with myelodysplastic syndromes (MDS). Findings suggest MDS may arise from a pluripotent stem cell, impacting leukemia development.

Keywords:

Refractory anemia early pre-B lymphoid transformation

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Last Updated: Mar 17, 2026

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Isolation of Precursor B-cell Subsets from Umbilical Cord Blood

Isolation of Precursor B-cell Subsets from Umbilical Cord Blood

Published on: April 16, 2013

Area of Science:

Hematology
Oncology
Stem Cell Biology

Background:

Myelodysplastic syndromes (MDS) are a group of clonal hematopoietic stem cell disorders.
Transformation to acute leukemia is a known complication of MDS, but lymphoblastic transformation is exceptionally rare.
Refractory anemia is a subtype of MDS often managed with supportive care.

Purpose of the Study:

To investigate the lineage of blast cells in a patient with refractory anemia experiencing acute transformation.
To contribute to the understanding of rare lymphoblastic transformations in the context of myelodysplastic syndromes.
To explore the clonal origin of myelodysplastic syndromes.

Main Methods:

Morphological and cytochemical studies to determine blast cell lineage.
Immunophenotypical analysis using monoclonal antibodies against various hematopoietic lineages (myeloid, monocytic, erythroid, megakaryocytic, lymphoid).
Analysis of immunoglobulin and T-cell receptor genes to confirm lymphoid origin.

Main Results:

Morphological and cytochemical studies were inconclusive in determining blast cell lineage.
Immunophenotyping and gene rearrangement studies definitively identified the blasts as early pre-B lymphoid in nature.
The patient presented with acute transformation six years after MDS diagnosis.

Conclusions:

Myelodysplastic syndromes are clonal disorders originating from a pluripotent stem cell.
Lymphoblastic transformation, though rare, can occur in myelodysplastic syndromes.
This case, along with similar reports, supports the pluripotent stem cell origin of MDS.