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A Droplet-Based Microfluidic Approach and Microsphere-PCR Amplification for Single-Stranded DNA Amplicons
Published on: November 14, 2018
Biophysical and chemical handles to control the size of DNA nanoparticles produced by rolling circle amplification
So Yeon Lee1, Kyoung-Ran Kim2, Duhee Bang3
1The Center for Theragnosis, Biomedical Research Institute, Korea Institute of Science and Technology (KIST), Hwarangno 14-gil 5, Seongbuk-gu, Seoul 136-791, Republic of Korea. drahn@kist.re.kr and Department of Chemistry, Korea University, 145 Anam-ro, Seongbuk-gu, Seoul, Republic of Korea.
Abstract:
Although rolling circle amplification (RCA) is an efficient method to produce DNA materials for biomedical applications, it does not yield nano-sized products suitable for intracellular delivery. We here provide the ways to control the size of RCA products and show a potential application of the size-controlled DNA nanoparticles.

