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Updated: Mar 17, 2026
Measurement of Tissue Non-Heme Iron Content using a Bathophenanthroline-Based Colorimetric Assay
Published on: January 31, 2022
Hematopoietic effect of fermented deer antler extract in iron deficient diet-induced anemic rats
Eun Young Jung1, Yooheon Park2, Moon Jea Woo3
1Department of Home Economic Education, Jeonju University, Jeonju, 55069, Republic of Korea.
Objective:
To assess the hematopoietic effects of fermented deer antler extract using a dietinduced anemic animal model to facilitate the utilization of fermented deer antler extract and its derived products.
Methods:
Thirty 3-week-old female Sprague-Dawley rats were treated for 5 weeks. The rats were randomly divided into 6 groups and treated as follows: control, saline; NFA200, non-fermented deer antler extract 200 mg/kg; NFA500, non-fermented deer antler extract 500 mg/kg; FAB200, fermented deer antler extract 200 mg/kg; FAB500, fermented deer antler extract 500 mg/kg; and PC, heme iron 0.2 mg/kg. Blood parameters, iron content in the liver and spleen, hepatic δ-aminolevulinic acid dehydrogenase (ALAD) activity and divalent metal transporter 1 (DMT1) mRNA expression were analyzed.
Results:
No detectable significant differences were observed in blood parameters among groups. The decrease in the hepatic ALAD activity in anemic rats was significantly improved by fermented deer antler extract supplementation (P<0.05); however, non-fermented deer antler extract supplementation did not result in a significant improvement (P>0.05). The hepatic DMT1 mRNA expression level was increased significantly by supplementation with both the fermented deer antler extract and the non-fermented deer antler extract in a dose-dependent manner compared with nontreatment in anemic rats (P<0.05).
Conclusion:
The hematopoietic activity induced by deer antler extract in dietinduced anemic rats might be increased through the fermentation process.
