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The expression of inhibitor of growth 4 is reduced in cervical cancer tissues

J Yuan1, Y-L Han, T-Y Wang

  • 1Department of Obstetrics and Gynecology, The First Affiliated Hospital of Harbin Medical University, Harbin, China. wangyan@hrbmu.edu.cn.

European Review for Medical and Pharmacological Sciences
|July 29, 2016
PubMed
Abstract

Insights

Inhibitor of growth 4 (ING4) is significantly decreased in cervical cancer tissues. Restoring ING4 expression reduces cancer cell proliferation and HPV E6/E7 transcription, suggesting its role in cervical cancer progression.

Area of Science:

  • Oncology
  • Molecular Biology
  • Cancer Research

Background:

  • Inhibitor of growth 4 (ING4) is a known tumor suppressor involved in DNA repair, apoptosis, cell cycle control, metastasis, and angiogenesis.
  • Limited clinical data exists on the specific role of ING4 in cervical cancer development and progression.

Purpose of the Study:

  • To investigate the expression levels of ING4 in cervical cancer tissues.
  • To analyze the correlation between ING4 expression and the clinical-pathological features of cervical cancer.
  • To explore the relationship between ING4 and human papillomavirus (HPV) E6/E7 transcription in cervical cancer cells.

Main Methods:

  • Quantitative real-time PCR (RT-PCR) and Western blot analysis were used to assess ING4 mRNA and protein levels.
  • Immunohistochemistry was employed to determine ING4 expression and subcellular localization in cervical cancer tissues.
  • Correlation analyses were performed with clinical-pathological features and HPV E6/E7 transcription levels in SiHa cells.

Main Results:

  • ING4 expression was significantly downregulated at both mRNA and protein levels in cervical cancer tissues compared to adjacent non-cancerous tissues.
  • Subcellular localization analysis revealed decreased ING4 expression in the nucleus of cervical cancer cells.
  • Ectopic expression of ING4 in SiHa cells led to reduced cell proliferation and decreased HPV E6/E7 transcription.

Conclusions:

  • ING4 expression is reduced in human cervical cancer tissues, indicating its potential as a tumor suppressor.
  • Reconstitution of ING4 expression in cervical cancer cells correlates with reduced HPV E6/E7 transcription.
  • These findings suggest that ING4 holds diagnostic and prognostic significance for cervical cancer.

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