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Updated: Mar 17, 2026

Comprehensive Workflow of Mass Spectrometry-based Shotgun Proteomics of Tissue Samples
Published on: November 13, 2021
Tissue-based quantitative proteome analysis of human hepatocellular carcinoma using tandem mass tags
Dominik Andre Megger1, Kristin Rosowski1, Maike Ahrens1
1a Medizinisches Proteom-Center, Ruhr-Universität Bochum , Germany.
Context And Objective:
Human hepatocellular carcinoma (HCC) is a severe malignant disease, and accurate and reliable diagnostic markers are still needed. This study was aimed for the discovery of novel marker candidates by quantitative proteomics.
Methods And Results:
Proteomic differences between HCC and nontumorous liver tissue were studied by mass spectrometry. Among several significantly upregulated proteins, translocator protein 18 (TSPO) and Ras-related protein Rab-1A (RAB1A) were selected for verification by immunohistochemistry in an independent cohort. For RAB1A, a high accuracy for the discrimination of HCC and nontumorous liver tissue was observed.
Conclusion:
RAB1A was verified to be a potent biomarker candidate for HCC.
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