Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Cells of the Adaptive Immune Response01:23

Cells of the Adaptive Immune Response

9.9K
The T and B lymphocytes of the adaptive immune system develop from common lymphoid progenitor cells in the bone marrow. These progenitors give rise to precursors that eventually develop into both T and B lymphocytes. As these precursors mature, they gain the ability to detect and respond to foreign antigens in the body, a process known as immunocompetence. Additionally, these precursors acquire self-tolerance, a process that ensures they do not react to self-antigens. This intricate system...
9.9K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Acute Lymphoblastic Leukaemia in Infancy: Clinical and Biological Features.

Leukemia & lymphoma·2016
Same author

Lack of awareness in both patients and physicians contributes to a high rate of late presentation in a South West German HIV patient cohort.

Infection·2015
Same author

[Metastatic bronchogenic epithelioma].

Revista mexicana de tuberculosis y enfermedades del aparato respiratorio·2014
Same author

[Pulmonary tuberculosis; streptomycin].

Revista mexicana de tuberculosis y enfermedades del aparato respiratorio·2014
Same author

[Associated dihydrostreptomycin and isonicotinic acid hydrazide in the treatment of pulmonary tuberculosis; preliminary communication].

Revista mexicana de tuberculosis y enfermedades del aparato respiratorio·2014
Same author

[Epidermoid bronchiogenic carcinoma; report of a case].

Revista mexicana de tuberculosis y enfermedades del aparato respiratorio·2014
Same journal

CPX‑351 vs. FLAG‑ida in patients with high-risk AML/MDS and MDS-related gene mutations: a subgroup analysis of the UK NCRI AML19 trial.

Leukemia & lymphoma·2026
Same journal

Specialist palliative care access and end-of-life hospitalizations in acute leukemia: a nationwide register study.

Leukemia & lymphoma·2026
Same journal

D-VRD vs. D-KRD induction in transplant-eligible multiple myeloma: a real-world comparison.

Leukemia & lymphoma·2026
Same journal

T-box factor EOMES is part of an aberrant network in Hodgkin lymphoma.

Leukemia & lymphoma·2026
Same journal

Overall survival of patients with chronic lymphocytic leukemia treated with venetoclax-obinutuzumab compared to age- and sex-matched general population.

Leukemia & lymphoma·2026
Same journal

Hypertension and time to treatment failure with acalabrutinib and ibrutinib in treatment-naïve CLL/SLL.

Leukemia & lymphoma·2026
See all related articles

Related Experiment Video

Updated: Mar 17, 2026

Establishment of Epstein-Barr Virus Growth-transformed Lymphoblastoid Cell Lines
06:38

Establishment of Epstein-Barr Virus Growth-transformed Lymphoblastoid Cell Lines

Published on: November 8, 2011

41.4K

An Early B Cell Line with a Variant 11;19 Translocation.

F Katz1,2, B Gibbons1,3, J Chessells2

  • 1a Imperial Cancer Research Fund, Institute for Child Health, London, WC1, UK.

Leukemia & Lymphoma
|July 29, 2016
PubMed
Summary
This summary is machine-generated.

A new cell line (BS) was established from infant acute lymphoblastic leukemia (ALL) with a unique 11;19 translocation. This cell line aids in studying leukemia translocation breakpoints.

Keywords:
B cell linevariant translocation

More Related Videos

An Efficient and Simple Method to Establish NK and T Cell Lines from Patients with Chronic Active Epstein-Barr Virus Infection
09:43

An Efficient and Simple Method to Establish NK and T Cell Lines from Patients with Chronic Active Epstein-Barr Virus Infection

Published on: March 30, 2018

9.8K
A Simple Red Blood Cell Lysis Method for the Establishment of B Lymphoblastoid Cell Lines
09:49

A Simple Red Blood Cell Lysis Method for the Establishment of B Lymphoblastoid Cell Lines

Published on: January 14, 2017

15.6K

Related Experiment Videos

Last Updated: Mar 17, 2026

Establishment of Epstein-Barr Virus Growth-transformed Lymphoblastoid Cell Lines
06:38

Establishment of Epstein-Barr Virus Growth-transformed Lymphoblastoid Cell Lines

Published on: November 8, 2011

41.4K
An Efficient and Simple Method to Establish NK and T Cell Lines from Patients with Chronic Active Epstein-Barr Virus Infection
09:43

An Efficient and Simple Method to Establish NK and T Cell Lines from Patients with Chronic Active Epstein-Barr Virus Infection

Published on: March 30, 2018

9.8K
A Simple Red Blood Cell Lysis Method for the Establishment of B Lymphoblastoid Cell Lines
09:49

A Simple Red Blood Cell Lysis Method for the Establishment of B Lymphoblastoid Cell Lines

Published on: January 14, 2017

15.6K

Area of Science:

  • Hematology
  • Oncology
  • Cell Biology

Background:

  • Acute lymphoblastic leukemia (ALL) in infants can present with complex chromosomal abnormalities.
  • Translocations involving chromosome 11 and 19 are recurrent in various leukemias.
  • Establishing cell lines with specific genetic alterations is crucial for leukemia research.

Purpose of the Study:

  • To establish and characterize a novel cell line (BS) from an infant with B-precursor ALL harboring a variant t(11;19) translocation.
  • To investigate the biological properties and potential differentiation capacity of the BS cell line.
  • To provide a valuable tool for studying the genetic basis of leukemia, particularly translocation breakpoints.

Main Methods:

  • Establishment of a cell line from infant ALL blast cells exposed to Epstein-Barr virus (EBV).
  • Morphological, cytochemical, and immunophenotypic analyses of the cell line and patient blasts.
  • Karyotyping to confirm the t(11;19) translocation.
  • Molecular analyses including immunoglobulin heavy chain rearrangement and screening for T cell receptor gene rearrangements.
  • Induction of differentiation using phorbol ester (PMA) and assessment of phenotypic changes.

Main Results:

  • The BS cell line exhibited morphology, cytochemistry, and immunophenotype consistent with early B-cell neoplasm, identical to the patient's blasts.
  • The cell line and patient blasts shared an identical karyotype: 46,XY,t(11;19)(q23;p13),t(11;19)(q13;q13).
  • Immunoglobulin heavy chain rearrangement was observed, but T cell receptor gene rearrangements were absent.
  • Exposure to PMA induced a myelomonocytic phenotype with co-expression of B and myeloid antigens.
  • No EBV nuclear antigens were detected, and the immortalization mechanism remains unclear.

Conclusions:

  • The BS cell line, with its unique karyotype and dual B-cell/myeloid potential, serves as a valuable model for studying leukemia.
  • It offers a unique opportunity to investigate the four translocation breakpoints frequently observed in acute lymphoblastic and acute myeloid leukemias.
  • Further research is needed to elucidate the EBV immortalization mechanism in this cell line.