Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Allosteric Proteins-ATCase01:19

Allosteric Proteins-ATCase

6.8K
Binding sites linkages can regulate a protein's function.  For example, enzyme activity is often regulated through a feedback mechanism where the end product of the biochemical process serves as an inhibitor.
Aspartate transcarbamoylase (ATCase) is a cytosolic enzyme that catalyzes the condensation of L-aspartate and carbamoyl phosphate to  N-carbamoyl-L-aspartate. This reaction is the first step in pyrimidine biosynthesis. UTP and CTP, the end products of the pyrimidine synthesis...
6.8K
ATP Synthase: Structure01:18

ATP Synthase: Structure

16.9K
ATP synthase or ATPase is among the most conserved proteins found in bacteria, mammals, and plants. This enzyme can catalyze a forward reaction in response to the electrochemical gradient, producing ATP from ADP and inorganic phosphate. ATP synthase can also work in a reverse direction by hydrolyzing ATP and generating an electrochemical gradient. Different forms of ATP synthases have evolved special features to meet the specific demands of the cell. Based on their specific feature, ATP...
16.9K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Guiding point-of-care therapeutic drug monitoring through structure-toxicity principles.

Chemical science·2026
Same author

Computer-Aided Rational Hapten Design for Broad-Spectrum Monoclonal Antibody Development against Anthraquinones and Its Application in Lateral Flow Immunoassay.

Analytical chemistry·2026
Same author

Fluorescence Polarization Immunoassay with Modulated Selectivity for Effective Detection of the Agrochemical 4-Chlorophenoxyacetic Acid.

Biosensors·2026
Same author

An ultrasensitive visual detection platform for forchlorfenuron: from simulation-assisted hapten and antibody production to double-T immunochromatographic assay application.

Journal of materials chemistry. B·2026
Same author

Colloidal gold immunochromatographic assay for mycophenolic acid in human plasma.

The Analyst·2026
Same author

A Multiplex Immunochromatographic Assay for the Simultaneous Detection of Nitroxynil and Oxyclozanide in Food and Environmental Samples.

Journal of agricultural and food chemistry·2026
Same journal

Scalable batch-type synthesis of layered 2D-SnS<sub>2</sub> transistors integration enabled by BEOL-compatible low-thermal budget processes.

Nanoscale·2026
Same journal

Self-powered and gate-reconfigurable photodetection and logic operations in the Ta<sub>2</sub>PdS<sub>6</sub>/WSe<sub>2</sub> van der Waals heterostructure.

Nanoscale·2026
Same journal

Elucidating interfacial charge extraction from CdTe@ZnS quantum dots by pyridinium ionic liquids.

Nanoscale·2026
Same journal

Tailoring charged nanochannels in covalent organic framework membranes for efficient lithium recovery.

Nanoscale·2026
Same journal

Restoring the powerhouse: mitochondrial transplantation in regenerative medicine and cancer therapy.

Nanoscale·2026
Same journal

Enhanced circular dichroism of molecular J-aggregate-surface plasmon polariton hybrid modes.

Nanoscale·2026
See all related articles

Related Experiment Video

Updated: Mar 17, 2026

A Semi-High-Throughput Adaptation of the NADH-Coupled ATPase Assay for Screening Small Molecule Inhibitors
10:28

A Semi-High-Throughput Adaptation of the NADH-Coupled ATPase Assay for Screening Small Molecule Inhibitors

Published on: August 17, 2019

10.5K

A self-assembled chiral-aptasensor for ATP activity detection.

Pan Fu1, Maozhong Sun1, Liguang Xu1

  • 1State Key Lab of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu 214122, People's Republic of China. kuangh@jiangnan.edu.cn and International Joint Research Laboratory for Biointerface and Biodetection, and School of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu 214122, People's Republic of China.

Nanoscale
|July 30, 2016
PubMed
Summary
This summary is machine-generated.

This study introduces a novel chiral aptasensor for detecting adenosine-5'-triphosphate (ATP) in living cells. The DNA-based gold nanoparticle sensor offers a simple, economical, and biocompatible platform for intracellular molecular probing.

More Related Videos

Mapping the Binding Site of an Aptamer on ATP Using MicroScale Thermophoresis
08:09

Mapping the Binding Site of an Aptamer on ATP Using MicroScale Thermophoresis

Published on: January 7, 2017

11.3K
An Aptamer-based Sensor for Unchelated GadoliniumIII
05:15

An Aptamer-based Sensor for Unchelated GadoliniumIII

Published on: January 9, 2017

7.8K

Related Experiment Videos

Last Updated: Mar 17, 2026

A Semi-High-Throughput Adaptation of the NADH-Coupled ATPase Assay for Screening Small Molecule Inhibitors
10:28

A Semi-High-Throughput Adaptation of the NADH-Coupled ATPase Assay for Screening Small Molecule Inhibitors

Published on: August 17, 2019

10.5K
Mapping the Binding Site of an Aptamer on ATP Using MicroScale Thermophoresis
08:09

Mapping the Binding Site of an Aptamer on ATP Using MicroScale Thermophoresis

Published on: January 7, 2017

11.3K
An Aptamer-based Sensor for Unchelated GadoliniumIII
05:15

An Aptamer-based Sensor for Unchelated GadoliniumIII

Published on: January 9, 2017

7.8K

Area of Science:

  • Nanomaterials Science
  • Biotechnology
  • Analytical Chemistry

Background:

  • Circular dichroism (CD) is crucial for chiral nanomaterial applications like biosensing.
  • A need exists for simple, target-specific, economical, and biocompatible platforms for intracellular detection and molecular probing.

Purpose of the Study:

  • To develop a DNA self-assembled chiral aptasensor using gold nanoparticle heterodimers for adenosine-5 -triphosphate (ATP) quantification.
  • To enable intracellular detection and in situ molecular probing of ATP within living cells.

Main Methods:

  • Self-assembly of a DNA zip-fastener structure with gold nanoparticle heterodimers.
  • Utilizing the interaction between ATP-conjugated aptamers and complementary DNA sequences on gold nanoparticles to alter CD signal.
  • Modification of nanoconjugates with cell-penetrating peptides (TAT) for intracellular delivery.

Main Results:

  • The chiral aptasensor demonstrated effective quantification of ATP via a decrease in CD signal.
  • The developed nanoconjugates exhibited low cytotoxicity and PEG-stability.
  • Intracellular ATP detection showed a linear range of 1.5–4.2 mM with a limit of detection (LOD) of 0.2 mM.

Conclusions:

  • The DNA-based chiral aptasensor provides a promising platform for quantifying ATP in living cells.
  • This chiroplasmonic sensor facilitates the investigation of biogenic biomolecules and their biological activity within cells and organisms.