Complete Genome Sequence of Bacillus subtilis subsp. subtilis Strain ∆6

Area of Science:

• Microbiology
• Genomics
• Synthetic Biology

Background:

• Bacillus subtilis is a key industrial microorganism.
• Genome reduction enhances Bacillus subtilis for biotechnological applications.
• The Bacillus subtilis ∆6 strain is a promising candidate for industrial use due to its reduced genome.

Purpose of the Study:

• To announce the complete genome sequence of Bacillus subtilis ∆6.
• To provide a genomic resource for further research and development.
• To investigate genetic elements within the Bacillus subtilis ∆6 genome.

Main Methods:

• Whole-genome sequencing of Bacillus subtilis ∆6.
• Bioinformatic analysis of the obtained genome sequence.
• Comparative genomics to identify genomic modifications.

Main Results:

• The full genome sequence of Bacillus subtilis ∆6 was determined.
• Bacillus subtilis ∆6 lacks six prophages and AT-rich islands.
• Evidence suggests the conjugative element ICEBs1 has undergone self-excision in this strain.

Conclusions:

• The genome sequence of Bacillus subtilis ∆6 is now available.
• This genome-reduced strain has significant potential for biotechnological applications.
• The absence or excision of ICEBs1 may contribute to the strain's stability and utility.