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Updated: Mar 17, 2026
09:06
MicroRNA Amplification and Recognition through Locked-nucleic-acid In situ Hybridization as a Novel Detection and Quantification Method
Published on: October 7, 2025
481
Circulating microRNAs in cancer: Hope or hype?
Rajbir Singh1, Brinda Ramasubramanian1, Suman Kanji1
1Department of Radiation Oncology, James Cancer Hospital and Solove Research Institute, The Ohio State University College of Medicine, Columbus, OH 43210, USA.
Cancer Letters
|July 30, 2016
Summary
Circulating microRNAs (miRNAs) show promise as disease biomarkers. However, their clinical utility requires further validation to confirm their diagnostic and prognostic significance.
Area of Science:
- Biochemistry
- Molecular Biology
- Genomics
Background:
- Circulating microRNAs (miRNAs) are stable, minimally invasive biomarkers with potential in disease diagnosis, prognosis, and treatment monitoring.
- Their release from producer cells and presence in body fluids offer new clinical avenues for targeted biomarker discovery.
Purpose of the Study:
- To critically review the ambiguous issues and challenges in the application of circulating miRNAs.
- To reevaluate the circulating miRNA domain for authenticating their specific roles and clinical significance.
Main Methods:
- Literature review and critical analysis of existing studies on circulating miRNAs.
- Discussion of discrepancies in reliability and response under physiological and pathological conditions.
Main Results:
- Circulating miRNAs are highly stable and detectable using common techniques like qPCR and microarray.
- Emerging discrepancies question their reliability and response in various conditions, with pending functional studies.
Conclusions:
- The clinical applicability of circulating miRNAs remains under deliberation due to unresolved issues.
- Further reevaluation and experimental validation are necessary to authenticate their specific roles and clinical weightage.
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