Raman spectroscopy detection of biomolecules in biocrusts from differing environmental conditions
I Miralles1, S E Jorge-Villar2, B van Wesemael3
1Earth and Life Institute, Université Catholique de Louvain, Louvain, -La-Neuve, Belgium; Experimental Station of Arid Zones (CSIC), Almería, Spain.
Summary
Species identity is the primary driver of biomolecule production in lichens and cyanobacteria. Environmental factors like microclimate and season have varied impacts, especially in cyanobacteria, with complex interactions influencing biomolecule synthesis.
Area of Science:
- Ecology
- Biochemistry
- Spectroscopy
Background:
- Lichens and cyanobacteria survive harsh environments by producing protective biomolecules.
- The influence of ecological factors on biomolecule synthesis in these organisms is not well understood.
Purpose of the Study:
- To investigate how species, microclimate, seasonality, and hydration state affect biomolecule production in lichens and cyanobacteria.
- To analyze the interactions between these ecological factors.
Main Methods:
- Raman Spectroscopy was used to detect and quantify biomolecules.
- Cyanobacterial biocrusts and three lichen species (Diploschistes diacapsis, Squamarina lentigera, Lepraria isidiata) were studied.
- Samples were analyzed across two microclimates, two seasons, and two hydration states.
Main Results:
- Species identity was the most significant factor determining biomolecule presence and frequency.
- Microclimate influenced biomolecules primarily in cyanobacteria, not significantly in all lichen species.
- Complex interactions were observed, with species-specific responses to microclimate and seasonality.
Conclusions:
- Biomolecule production in lichens and cyanobacteria is largely species-dependent.
- Environmental factors have differential and interactive effects on biomolecule synthesis.
- Raman spectrometry is a valuable tool for monitoring biomolecules in biocrusts.
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