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Low-coherence wavelength shifting interferometry for high-speed quantitative phase imaging.

Shichao Chen, Chengshuai Li, Yizheng Zhu

|July 30, 2016

Summary

This summary is machine-generated.

We developed low-coherence wavelength shifting interferometry for high-speed, sensitive quantitative phase imaging. This technique accurately measures dynamic biological samples, like sperm cells, in real-time.

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Area of Science:

Biomedical Optics
Interferometry
Quantitative Phase Imaging

Background:

Quantitative phase imaging (QPI) is crucial for label-free, high-resolution microscopy.
Dynamic biological processes require high-speed imaging capabilities.
Existing QPI methods face limitations in speed and sensitivity for dynamic specimens.

Purpose of the Study:

To introduce and validate a novel low-coherence wavelength shifting interferometry technique.
To enable high-sensitivity, high-speed quantitative phase imaging of dynamic specimens.
To demonstrate the application of this method for real-time analysis of biological samples.

Main Methods:

Acquisition of multiple interferograms by shifting the source wavelength across different spectral bands.
Phase extraction using a modified Carré algorithm for four-band imaging.
Implementation with a swept laser-based Mach-Zehnder interferometer for real-time data capture.

Main Results:

Successful demonstration of real-time imaging of live sperm cells at 62.5 Hz.
Accurate measurement of the dynamic dry mass of sperm heads.
Achieved a full-scale error of ±2%, confirming high sensitivity and accuracy.

Conclusions:

Low-coherence wavelength shifting interferometry is a viable technique for high-speed QPI.
The method offers high sensitivity for measuring dynamic changes in biological samples.
This technique advances the capabilities for real-time, quantitative analysis in live-cell microscopy.