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Related Experiment Video

Updated: Mar 17, 2026

An Organotypic Slice Assay for High-Resolution Time-Lapse Imaging of Neuronal Migration in the Postnatal Brain
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Visualizing septin and cell dynamics in mammalian brain slices.

H Ito1, R Morishita1, H Tabata1

  • 1Aichi Human Service Center, Kasugai, Japan.

Methods in Cell Biology
|July 31, 2016
PubMed
Summary

Correct neuronal migration is vital for brain development. This study details in utero electroporation (IUE) and in vivo electroporation (IVE) methods for analyzing gene function in brain development and disorders.

Keywords:
Cerebral cortexCorticogenesisHippocampusIn utero electroporationIn vivo electroporationSeptin

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Related Experiment Videos

Last Updated: Mar 17, 2026

An Organotypic Slice Assay for High-Resolution Time-Lapse Imaging of Neuronal Migration in the Postnatal Brain
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Area of Science:

  • Neuroscience
  • Developmental Biology
  • Genetics

Background:

  • Neuronal migration is essential for proper brain architecture and function.
  • Excitatory and inhibitory neurons migrate during development from specific progenitor zones.
  • Accurate spatiotemporal regulation of migration is critical.

Purpose of the Study:

  • To describe and exemplify advanced methods for studying brain development.
  • To showcase the utility of in utero electroporation (IUE) and in vivo electroporation (IVE) for gene function analysis.
  • To highlight the role of septins in neuronal development and neurological disorders.

Main Methods:

  • Basic immunohistochemistry for visualizing protein distribution (e.g., Sept6, Sept9, Sept14).
  • In utero electroporation (IUE) for gene transfer into rodent embryonic progenitor cells.
  • In vivo electroporation (IVE) for gene transfer into neonatal mouse hippocampal granule cells.
  • IUE-based time-lapse imaging for analyzing cortical neuron migration.

Main Results:

  • Demonstrated immunohistochemistry for septin distribution.
  • Provided examples of IUE for functional analysis of Sept14 during brain development.
  • Illustrated IVE for gene transfer in the hippocampus.
  • Explained IUE-based time-lapse imaging for migration studies.

Conclusions:

  • IUE and IVE are powerful tools for gain- and loss-of-function studies in brain development.
  • These methods facilitate morphological analysis of molecules like septins.
  • Understanding neuronal migration mechanisms is key to elucidating roles in neurological and psychiatric disorders.