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Septins

Septins

Septins are protein filaments forming the cytoskeleton along with the microtubules, microfilaments, intermediate filaments, and other accessory proteins. In 1971 while studying the cell division cycle in mutant Saccharomyces cerevisiae Harwell et al. first identified the septin-related genes playing a crucial role in yeast cytokinesis. Fluorescence microscopy revealed that these proteins localize at the budding neck as rings. These ring-like proteins were then named Septins by John Pringle, and...

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Related Experiment Video

Updated: Mar 2, 2026

Reconstitution of Septin Assembly at Membranes to Study Biophysical Properties and Functions

Reconstitution of Septin Assembly at Membranes to Study Biophysical Properties and Functions

Published on: July 28, 2022

Septin crystallization for structural analysis.

N F Valadares¹, R C Garratt¹

¹University of Brasília, Brasília, Brazil; University of São Paulo, São Carlos, Brazil.

Methods in Cell Biology

|July 31, 2016

Summary

This summary is machine-generated.

Septins are crucial cytoskeleton proteins. This study reviews methods to overcome experimental challenges in septin crystallography, aiding research on cell division and membrane dynamics.

Keywords:

Construct Crystallization Crystallography Protein structure Sample preparation Septins

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Last Updated: Mar 2, 2026

Reconstitution of Septin Assembly at Membranes to Study Biophysical Properties and Functions

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Published on: July 28, 2022

Purification and Quality Control of Recombinant Septin Complexes for Cell-Free Reconstitution

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Published on: June 23, 2022

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Area of Science:

Biochemistry
Cell Biology
Structural Biology

Background:

Septins are essential eukaryotic filament-forming cytoskeletal proteins.
Understanding septin macromolecular assemblies and crystal structures is vital for cellular processes like cell division and membrane dynamics.
Experimental difficulties often hinder the acquisition of high-quality septin structural data.

Purpose of the Study:

To provide an overview of current approaches to overcome experimental challenges in septin crystallography.
To focus on optimizing septin construct selection and sample preparation for successful crystallization.

Main Methods:

Review of existing literature and experimental strategies for septin structural studies.
Emphasis on protein construct design and purification techniques tailored for septins.
Discussion of common pitfalls and solutions in septin sample preparation for crystallography.

Main Results:

Identification of key experimental hurdles in septin crystallography.
Presentation of strategies for selecting optimal septin constructs.
Guidance on effective sample preparation methods to improve crystallization outcomes.

Conclusions:

Overcoming experimental challenges is key to advancing septin structural biology.
Careful selection of septin constructs and meticulous sample preparation are critical for successful crystallography.
This work facilitates further research into septin functions and their roles in cellular processes.