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Updated: Mar 17, 2026

Purification of the Cystic Fibrosis Transmembrane Conductance Regulator Protein Expressed in Saccharomyces cerevisiae
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Purification of the Cystic Fibrosis Transmembrane Conductance Regulator Protein Expressed in Saccharomyces cerevisiae

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Trafficking and function of the cystic fibrosis transmembrane conductance regulator: a complex network of

Michelle L McClure1, Stephen Barnes2, Jeffrey L Brodsky3

  • 1Cystic Fibrosis Research Center, University of Alabama at Birmingham, Birmingham, Alabama.

American Journal of Physiology. Lung Cellular and Molecular Physiology
|July 31, 2016
PubMed

Insights

Cystic fibrosis transmembrane conductance regulator (CFTR) protein modifications regulate its function and stability. Understanding these posttranslational modifications (PTMs) may lead to new therapeutic strategies for CF.

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Background:

  • Posttranslational modifications (PTMs) are crucial for protein function diversity.
  • The cystic fibrosis transmembrane conductance regulator (CFTR) protein is subject to numerous PTMs throughout its lifecycle.

Purpose of the Study:

  • To comprehensively review co- and posttranslational CFTR modifications.
  • To highlight the significance of CFTR PTMs in protein biogenesis.

Main Methods:

  • Literature review of CFTR PTMs.
  • Analysis of the impact of PTMs on CFTR biogenesis.

Main Results:

  • CFTR undergoes various PTMs including glycosylation, ubiquitination, sumoylation, phosphorylation, and palmitoylation.
  • These modifications influence CFTR folding, trafficking, stability, function, and interactions.
  • PTMs are critical regulatory steps in CFTR protein biogenesis.

Conclusions:

  • Understanding CFTR PTMs offers potential therapeutic targets for cystic fibrosis.
  • Insights into CFTR PTMs may inform treatments for other protein conformational diseases.
Keywords:
cystic fibrosisglycosylationpalmitoylationphosphorylationprotein traffickingubiquitination

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