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Xyloglucans from flaxseed kernel cell wall: Structural and conformational characterisation.

Huihuang H Ding1, Steve W Cui1, H Douglas Goff2

  • 1Department of Food Science, University of Guelph, 50 Stone Road E., Guelph N1 G 2W1, Canada; Guelph Research and Development Centre, Agriculture and Agri-Food Canada, 93 Stone Road W., Guelph N1 G 5C9, Canada.

Carbohydrate Polymers
|July 31, 2016
PubMed
Summary

Flaxseed kernel polysaccharides (KPI-EPF) were analyzed, revealing a highly branched xyloglucan structure. These findings enhance understanding of flaxseed cell walls and potential applications.

Keywords:
ConformationDietary fibreFlaxseedStructureXyloglucan

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Area of Science:

  • Plant Biochemistry
  • Food Science
  • Carbohydrate Chemistry

Background:

  • Flaxseed kernel cell wall polysaccharides are complex and require detailed structural elucidation.
  • Understanding polysaccharide structure is key to unlocking their functional properties and applications.

Purpose of the Study:

  • To determine the molecular structure of ethanol precipitated fraction from flaxseed kernel polysaccharides (KPI-EPF).
  • To characterize KPI-EPF as a potential xyloglucan for various applications.

Main Methods:

  • Methylation analysis coupled with Gas Chromatography-Mass Spectrometry (GC-MS).
  • Nuclear Magnetic Resonance (NMR) spectroscopy.
  • Matrix-Assisted Laser Desorption/Ionization-Time of Flight Mass Spectrometry (MALDI-TOF-MS).
  • Static Light Scattering (SLS) for molecular weight determination.

Main Results:

  • KPI-EPF is primarily composed of (1,4,6)-linked-β-d-glucopyranose (24.1mol%), terminal α-d-xylopyranose (16.2mol%), and other xylose, glucose, and galactose residues.
  • The structure was identified as xyloglucans with a high backbone substitution rate (69.3%) and complex branching patterns.
  • The molecular weight (Mw) was determined to be 1506 kDa, with a structure-sensitive parameter (ρ) of 1.44 indicating a highly branched structure.

Conclusions:

  • The study successfully elucidated the complex xyloglucan structure of KPI-EPF from flaxseed kernels.
  • The findings provide valuable insights into flaxseed cell wall composition and the properties of its polysaccharides.
  • This detailed structural information will aid in exploring the fermentation properties and potential industrial applications of flaxseed xyloglucans.