Related Experiment Video

Updated: Mar 17, 2026

Production of Xenopus tropicalis Egg Extracts to Identify Microtubule-associated RNAs
10:01

Production of Xenopus tropicalis Egg Extracts to Identify Microtubule-associated RNAs

Published on: June 27, 2013

14.3K

Spatial control of translation repression and polarized growth by conserved NDR kinase Orb6 and RNA-binding protein

Illyce Nuñez1, Marbelys Rodriguez Pino1, David J Wiley1

  • 1Molecular and Cellular Pharmacology, University of Miami School of Medicine, Miami, United States.

Elife
|July 31, 2016
PubMed

Insights

Fission yeast Orb6 kinase controls cell growth by regulating RNA-binding protein Sts5. Orb6 kinase limits Sts5 recruitment into RNP granules, enabling polarized cell growth and normal cell morphology.

Area of Science:

  • Cell Biology
  • Molecular Biology
  • Genetics

Background:

  • RNA-binding proteins form ribonucleoprotein (RNP) granules via phase transition.
  • The fission yeast kinase Orb6 regulates cell morphogenesis by controlling Cdc42 GTPase.
  • Mechanisms governing RNP granule formation and function are not fully elucidated.

Purpose of the Study:

  • To investigate a novel function of Orb6 kinase in regulating RNA-binding proteins.
  • To understand Orb6's role in the spatial control of RNP granule formation and polarized cell growth.

Main Methods:

  • Utilized fission yeast as a model organism.
  • Investigated the interaction between Orb6 kinase and RNA-binding protein Sts5.
  • Analyzed the impact of Orb6 on Sts5 localization, RNP granule formation, and mRNA degradation.

Main Results:

  • Orb6 kinase negatively regulates the recruitment of Sts5 into RNP granules and processing bodies.
  • Orb6 promotes Sts5 interaction with the 14-3-3 protein Rad24, inhibiting Sts5 function.
  • Orb6 kinase controls the spatial and temporal extent of Sts5 granule formation, affecting the degradation of Sts5-bound mRNAs.
  • Disruption of Orb6-Sts5 regulation impairs cell morphology and polarized growth patterns.

Conclusions:

  • Orb6 kinase plays a crucial role in negatively regulating Sts5 recruitment into RNP granules.
  • This regulation by Orb6 is essential for promoting polarized cell growth and normal cell morphogenesis in fission yeast.
  • Orb6 kinase mediates spatial control of translational repression through Sts5 granules, impacting cell growth and development.
Keywords:
14-3-3 proteinNDR kinaseP-bodyRNP granule assemblyS. pombecell biologypolarized growthtranslational repression

More Related Videos

Detection of Axonally Localized mRNAs in Brain Sections Using High-Resolution In Situ Hybridization11:24

Detection of Axonally Localized mRNAs in Brain Sections Using High-Resolution In Situ Hybridization

Published on: June 17, 2015

12.3K
Spatio-Temporal Manipulation of Small GTPase Activity at Subcellular Level and on Timescale of Seconds in Living Cells10:27

Spatio-Temporal Manipulation of Small GTPase Activity at Subcellular Level and on Timescale of Seconds in Living Cells

Published on: March 9, 2012

11.3K

Related Concept Videos

Regulation of Nuclear Protein Sorting01:45

Regulation of Nuclear Protein Sorting

Nuclear protein sorting regulates nucleus composition and gene expression, crucial for determining the fate of a eukaryotic cell. Hence, the entry and exit of molecules across the nuclear envelope is a tightly controlled process. Nuclear protein sorting can be inhibited by one of the following ways: 1) masking cargo signal sequences, 2) modifying the nuclear receptor's affinity for cargo, 3) controlling the nuclear pore size, 4) retaining the cargo during its transit to the cytosol or the...
3.4K
Translational Regulation01:29

Translational Regulation

Translational regulation in prokaryotes ensures efficient protein synthesis by controlling ribosome access to mRNA. This regulation is mediated by secondary RNA structures, including translational riboswitches, RNA thermometers, and small RNAs (sRNAs), which respond to intracellular and environmental signals to modulate gene expression.Translational RiboswitchesRiboswitches in the leader region of mRNAs can regulate translation by altering the accessibility of the Shine-Dalgarno (SD) sequence,...
759
Regulation of Expression at Multiple Steps01:23

Regulation of Expression at Multiple Steps

The gene expression in cells is regulated at different stages: (i) transcription, (ii) RNA processing, (iii) RNA localization, and (iv) translation. Transcriptional regulation is mediated by regulatory proteins such as transcription factors, activators, or repressors—these control gene expression by initiating or inhibiting the transcription of genes. Once a precursor or pre-mRNA is produced, it undergoes post-transcriptional modification, including 5' capping, splicing, and the...
1.5K
Regulated mRNA Transport02:22

Regulated mRNA Transport

In eukaryotes, transcription and translation are compartmentalized; an mRNA is first synthesized in the nucleus and then selectively transported to the cytoplasm for protein synthesis. Before transport, a pre-mRNA undergoes several steps of post-transcriptional modifications including splicing, 5' capping, and the addition of a poly-adenine tail. Various proteins bind to the pre-mRNA during these modifications. The mRNA transport takes place with the help of multiple proteins playing...
7.1K
Regulated mRNA Transport02:22

Regulated mRNA Transport

3.5K
Regulation of Expression Occurs at Multiple Steps02:24

Regulation of Expression Occurs at Multiple Steps

Gene expression can be regulated at almost every step from gene to protein. Transcription is the step that is most commonly regulated. This involves the binding of proteins to short regulatory sequences on the DNA. This association can either promote or inhibit the transcription of a gene associated with the respective sequence.
Transcription results in the generation of precursor (pre-mRNA) that consists of both exons and introns, which needs further processing before being translated to a...
26.8K