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Updated: Mar 17, 2026
Antigen-Capture Enzyme-Linked Immunosorbent Assay for Specific Detection of Mycoplasma pneumoniae
Published on: February 24, 2023
Serum cytokine profile contributes to discriminating M. pneumoniae pneumonia in children
Xue-Feng Xu1, Xue-Jing Li1, Jin-Ling Liu1
1Department of Respiratory Medicine, The Children's Hospital, Zhejiang University School of Medicine, Hangzhou, China.
Background:
To evaluate the role of serum cytokines in discriminating M. pneumoniae infection in children with community-acquired pneumonia (CAP).
Methods:
A prospective observational study was conducted. 385 hospitalized patients with CAP had only M. pneumoniae (MP group) infection; 321 hospitalized patients with CAP had no M. pneumoniae and other specific pathogen (control group) infections. Serum interleukin-2 (IL-2), IL-4, IL-6, IL-10, tumor necrosis factor-α (TNF-α), and interferon-γ (IFN-γ) were detected by flow cytometry.
Results:
In children younger than 5years, serum IL-6, TNF-α, and IFN-γ levels from MP group were significantly higher than those from control group. However in children 5-15years, serum IL-6, IL-10, and IFN-γ levels from MP group were significantly higher than those from control group. In the final multivariate logistic regression model for serum cytokine, moderately elevated IL-6, IL-10, and IFN-γ shows a higher prediction of development of M. pneumoniae pneumonia among CAP patients.
Conclusions:
A specific cytokine pattern showed a higher prediction of M. pneumoniae pneumonia among CAP patients, further suggesting that serum cytokine pattern might be useful in differentiating infectious causative agents in children.
Insights
Serum cytokine patterns, including interleukin-6 (IL-6), interleukin-10 (IL-10), and interferon-gamma (IFN-γ), can help predict Mycoplasma pneumoniae pneumonia in children with community-acquired pneumonia (CAP). This finding aids in differentiating infectious agents.
Area of Science:
- Pediatric Infectious Diseases
- Immunology
- Respiratory Medicine
Background:
- Community-acquired pneumonia (CAP) in children can be caused by various pathogens, including Mycoplasma pneumoniae (MP).
- Accurate and timely diagnosis of MP pneumonia is crucial for appropriate treatment and management.
- Distinguishing MP from other causes of CAP can be challenging.
Purpose of the Study:
- To investigate the role of serum cytokines in differentiating Mycoplasma pneumoniae infections in pediatric CAP patients.
- To identify specific cytokine profiles associated with MP pneumonia.
Main Methods:
- A prospective observational study involving 385 children with MP CAP (MP group) and 321 children with non-MP CAP (control group).
- Serum levels of interleukin-2 (IL-2), IL-4, IL-6, IL-10, tumor necrosis factor-α (TNF-α), and interferon-γ (IFN-γ) were measured using flow cytometry.
- Multivariate logistic regression analysis was employed to assess the predictive value of cytokines.
Main Results:
- In children younger than 5 years, significantly higher levels of IL-6, TNF-α, and IFN-γ were observed in the MP group compared to the control group.
- In children aged 5-15 years, significantly higher levels of IL-6, IL-10, and IFN-γ were found in the MP group.
- Elevated IL-6, IL-10, and IFN-γ showed a higher prediction for developing M. pneumoniae pneumonia among CAP patients.
Conclusions:
- A distinct serum cytokine pattern can predict M. pneumoniae pneumonia in children with CAP.
- Serum cytokine profiles may serve as a valuable tool for differentiating infectious agents in pediatric pneumonia.
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