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Human t-DARPP is induced during striatal development.

Marco Straccia1, Jordi Carrere1, Anne E Rosser2

  • 1Laboratory of Stem Cells and Regenerative Medicine, Department of Biomedical Sciences, Faculty of Medicine and Health Sciences, University of Barcelona, Barcelona, Spain; August Pi i Sunyer Biomedical Research Institute (IDIBAPS), Barcelona, Spain; Networked Biomedical Research Centre for NeuroDegenerative Disorders (CIBERNED), Spain.

Neuroscience
|August 1, 2016
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Summary
This summary is machine-generated.

Human Dopamine- and cAMP-regulated phosphoprotein (DARPP-32) isoforms show differential expression during striatal development. The truncated DARPP-32 isoform is absent in fetal samples and highly induced in the adult human striatum.

Keywords:
DARPP-32Huntington’s diseasehuman developmentmedium spiny neuronsneurodevelopmentstriatum

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Area of Science:

  • Neuroscience
  • Molecular Biology
  • Developmental Biology

Background:

  • DARPP-32 (PPP1R1B) protein has two isoforms: full-length (fl) and truncated (t).
  • fl-DARPP-32 is crucial in the central nervous system for integrating neurotransmitter signals in medium spiny neurons (MSNs).
  • Limited data exists on human DARPP-32 isoform expression during MSN maturation.

Purpose of the Study:

  • To investigate the differential expression of human DARPP-32 isoforms during striatal development.
  • To assess the performance of common anti-DARPP-32 antibodies in recognizing these isoforms in fetal and adult human striatal samples.

Main Methods:

  • Western blot analysis of human fetal and adult striatal samples.
  • Immunohistochemistry using four common anti-DARPP-32 antibodies.
  • Comparison of antibody recognition of fl-DARPP-32 and t-DARPP isoforms.

Main Results:

  • DARPP-32 isoform expression is differentially regulated during human striatal development.
  • The t-DARPP isoform is virtually absent in fetal whole ganglionic eminence (WGE) but highly induced in the adult striatum (caudate and putamen).
  • All four tested antibodies specifically recognized fl-DARPP-32 in both fetal and adult samples, while t-DARPP was only detected in adult samples.

Conclusions:

  • Human DARPP-32 protein isoform expression is dependent on the striatal neurodevelopmental stage.
  • The t-DARPP isoform is specific to the human adult striatum.
  • Antibody selection is critical for accurate detection of DARPP-32 isoforms in developmental studies.