Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Interfacial Electrochemical Methods: Overview01:06

Interfacial Electrochemical Methods: Overview

1.0K
Interfacial electrochemical methods focus on the phenomena occurring at the boundary between an electrode and a solution, as opposed to bulk methods that concentrate on the solution's overall properties. These interfacial methods are classified as either static or dynamic based on the presence of a nonzero current in the electrochemical cell and the consistency of analyte concentrations. Static methods, such as potentiometry, measure the cell's potential without any significant current...
1.0K
Voltammetric Techniques: Pulse Voltammetry01:17

Voltammetric Techniques: Pulse Voltammetry

1.8K
Differential-pulse voltammetry (DPV) is a type of voltammetry that involves applying a series of voltage pulses to an electrochemical cell while measuring the resulting current. In DPV, the differential pulse or small potential pulses are superimposed on a linear potential sweep. The magnitude of these pulses is typically small, often in the millivolt range. Each voltage pulse lasts a short duration, usually in the order of a few milliseconds, and is applied at regular intervals along the...
1.8K
Voltammetric Techniques: Linear-Scan (E vs Time)01:12

Voltammetric Techniques: Linear-Scan (E vs Time)

1.4K
Polarography is a classical voltammetric technique used to analyze electrochemical reactions. This method applies a linear potential sweep to a dropping mercury electrode (DME), and the resulting current is measured. A dropping mercury electrode is commonly used as the working electrode in polarography. It consists of a capillary tube filled with mercury, where the tiny droplet forms at the tip. This droplet continuously drops from the capillary, creating a new electrode surface for each...
1.4K
Electrochemical Systems01:24

Electrochemical Systems

51
Electrochemical systems provide a fascinating insight into the dynamic interplay of charged species within various phases. One notable example is the interaction between a membrane permeable to K⁺ ions but not to Cl⁻ ions, separating an aqueous KCl solution from pure water. As K⁺ ions diffuse through the membrane, they generate net charges on each phase, leading to a potential difference between them.Similarly, when a piece of Zn is immersed in an aqueous ZnSO₄ solution,...
51
Voltammetric Techniques: Cyclic Voltammetry01:10

Voltammetric Techniques: Cyclic Voltammetry

1.8K
Cyclic voltammetry (CV) is an electrochemical technique used to investigate the redox properties of a chemical species. It involves measuring the current response of an electrochemical cell as a function of the applied potential. The setup for cyclic voltammetry typically consists of a working electrode, a reference electrode, and a counter electrode—all immersed in an electrolyte solution. The working electrode is where the redox reaction of interest occurs, while the reference electrode...
1.8K
Voltammetry: Stripping Methods01:13

Voltammetry: Stripping Methods

1.2K
Anodic Stripping Voltammetry (ASV), Cathodic Stripping Voltammetry (CSV), and Adsorptive Stripping Voltammetry (AdSV) are electrochemical techniques used to determine trace amounts of analytes in solution. These methods involve applying a potential to an electrode and measuring the resulting current.
Anodic Stripping Voltammetry (ASV)
ASV is used to determine metals and metalloids at trace levels. It involves two steps: deposition and stripping. First, a negative potential is applied to the...
1.2K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Crystallise, poise, capture: a multimodal platform for correlated structural and spectroscopic characterisation of redox enzymes.

Journal of biological inorganic chemistry : JBIC : a publication of the Society of Biological Inorganic Chemistry·2026
Same author

Engineering the Electron Relay in [FeFe]-Hydrogenase Enhances Electrocatalytic H<sub>2</sub> Evolution.

ACS catalysis·2025
Same author

Reversible Enzymatic Switching of the Oxidation State of a Eu<sup>III/II</sup> Complex Controls Relaxivity.

Journal of the American Chemical Society·2025
Same author

Cyanophenylalanine as an Infrared Probe for Iron-Sulfur Cluster Redox State in Multicenter Metalloenzymes.

Chembiochem : a European journal of chemical biology·2025
Same author

LnDOTA Releasing Probes for Luminescence and Magnetic Resonance Imaging.

Inorganic chemistry·2025
Same author

Exploiting hydrogenases for biocatalytic hydrogenations.

Chemical communications (Cambridge, England)·2024

Related Experiment Video

Updated: Mar 17, 2026

Raman and IR Spectroelectrochemical Methods as Tools to Analyze Conjugated Organic Compounds
09:11

Raman and IR Spectroelectrochemical Methods as Tools to Analyze Conjugated Organic Compounds

Published on: October 12, 2018

19.1K

Vibrational Spectroscopic Techniques for Probing Bioelectrochemical Systems.

Philip A Ash1, Kylie A Vincent2

  • 1Department of Chemistry, Inorganic Chemistry Laboratory, University of Oxford, South Parks Road, Oxford, OX1 3QR, UK.

Advances in Biochemical Engineering/Biotechnology
|August 1, 2016
PubMed
Summary

Vibrational spectroscopies like infrared and Raman offer unique insights into bioelectrochemical interfaces. These methods provide real-time structural information of electrode-immobilized proteins, advancing both fundamental science and biotechnology.

Keywords:
BiocatalysisElectrocatalysisIn situ spectroscopyInfraredMembrane proteinRamanRedox enzymeSEIRASERRSSERSSpectroelectrochemistry

More Related Videos

Characterizing Mediated Extracellular Electron Transfer in Lactic Acid Bacteria with a Three-Electrode, Two-Chamber Bioelectrochemical System
10:23

Characterizing Mediated Extracellular Electron Transfer in Lactic Acid Bacteria with a Three-Electrode, Two-Chamber Bioelectrochemical System

Published on: August 23, 2024

2.0K
Using Cyclic Voltammetry, UV-Vis-NIR, and EPR Spectroelectrochemistry to Analyze Organic Compounds
11:44

Using Cyclic Voltammetry, UV-Vis-NIR, and EPR Spectroelectrochemistry to Analyze Organic Compounds

Published on: October 18, 2018

27.8K

Related Experiment Videos

Last Updated: Mar 17, 2026

Raman and IR Spectroelectrochemical Methods as Tools to Analyze Conjugated Organic Compounds
09:11

Raman and IR Spectroelectrochemical Methods as Tools to Analyze Conjugated Organic Compounds

Published on: October 12, 2018

19.1K
Characterizing Mediated Extracellular Electron Transfer in Lactic Acid Bacteria with a Three-Electrode, Two-Chamber Bioelectrochemical System
10:23

Characterizing Mediated Extracellular Electron Transfer in Lactic Acid Bacteria with a Three-Electrode, Two-Chamber Bioelectrochemical System

Published on: August 23, 2024

2.0K
Using Cyclic Voltammetry, UV-Vis-NIR, and EPR Spectroelectrochemistry to Analyze Organic Compounds
11:44

Using Cyclic Voltammetry, UV-Vis-NIR, and EPR Spectroelectrochemistry to Analyze Organic Compounds

Published on: October 18, 2018

27.8K

Area of Science:

  • Biochemistry
  • Electrochemistry
  • Spectroscopy

Background:

  • Understanding bioelectrochemical interfaces is crucial for protein studies and biotechnology.
  • Bioelectrochemical methods offer activity data, but lack in situ structural insights.
  • Spectroscopic techniques are needed for direct structural information of proteins at interfaces.

Purpose of the Study:

  • To highlight the application of infrared and Raman spectroscopies for studying electrode-immobilized proteins.
  • To demonstrate the utility of vibrational spectroscopy in bioelectrochemistry.

Main Methods:

  • Simultaneous electrochemical and spectroscopic data collection from the same electrode.
  • Application of in situ infrared and Raman spectroscopies.
  • Study of electrode-immobilized protein species.

Main Results:

  • Vibrational spectroscopies provide direct structural 'snapshots' of protein function.
  • These methods allow probing of metal coordination, protonation, cofactor changes, and protein structure.
  • Simultaneous, rapid, room-temperature analysis is achievable.

Conclusions:

  • Vibrational spectroscopic approaches are uniquely suited for bioelectrochemical studies.
  • These techniques offer comprehensive insights into protein behavior at electrode interfaces.
  • Advancements in understanding bioelectrochemical interfaces through spectroscopy.