Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Electron Microscope Tomography and Single-particle Reconstruction01:07

Electron Microscope Tomography and Single-particle Reconstruction

3.0K
Transmission electron microscopy (TEM) can be used to determine the 3D structure of biological samples with the help of techniques such as electron microscope tomography and single-particle reconstruction. While single-particle reconstruction can examine macromolecules and macromolecular complexes in vitro conditions only, tomography permits the study of cell components or small cells in vivo.
Electron Tomography
Electron tomography can be performed either in TEM or STEM (scanning transmission...
3.0K
Transmission Electron Microscopy01:15

Transmission Electron Microscopy

7.7K
In 1931, physicist Ernst Ruska—building on the idea that magnetic fields can direct an electron beam just as lenses can direct a beam of light in an optical microscope—developed the first prototype of the electron microscope. This development led to the development of the field of electron microscopy. In the transmission electron microscope (TEM), electrons are produced by a hot tungsten element and accelerated by a potential difference in an electron gun, which gives them up to 400...
7.7K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Early deformation mechanisms in the shear affected region underneath a copper sliding contact.

Nature communications·2020
Same author

Challenges in quantitative crystallographic characterization of 3D thin films by ACOM-TEM.

Ultramicroscopy·2016
Same author

Potassium polytitanate gas-sensor study by impedance spectroscopy.

Analytica chimica acta·2015
Same author

Combination of in situ straining and ACOM TEM: a novel method for analysis of plastic deformation of nanocrystalline metals.

Ultramicroscopy·2013
Same author

Accurate segmentation of dense nanoparticles by partially discrete electron tomography.

Ultramicroscopy·2012
Same author

Strain relaxation and vacancy creation in thin platinum films.

Physical review letters·2012

Related Experiment Video

Updated: Mar 17, 2026

Analysis of Minerals Produced by hFOB 1.19 and Saos-2 Cells Using Transmission Electron Microscopy with Energy Dispersive X-ray Microanalysis
14:55

Analysis of Minerals Produced by hFOB 1.19 and Saos-2 Cells Using Transmission Electron Microscopy with Energy Dispersive X-ray Microanalysis

Published on: June 24, 2018

9.8K

Comprehensive analysis of TEM methods for LiFePO4/FePO4 phase mapping: spectroscopic techniques (EFTEM, STEM-EELS)

X Mu1, A Kobler2, D Wang3

  • 1Institute of Nanotechnology, Karlsruhe Institute of Technology, 76344 Eggenstein-Leopoldshafen, Germany; Helmholtz-Institute Ulm for Electrochemical Energy Storage (HIU), Karlsruhe Institute of Technology (KIT), 89081 Ulm, Germany.

Ultramicroscopy
|August 1, 2016
PubMed
Summary

This study validates multiple Transmission Electron Microscopy techniques for mapping lithium distribution in lithium iron phosphate (LFP) battery materials. Spectroscopic and STEM diffraction methods reliably map lithiation, crucial for advanced Li-ion battery development.

Keywords:
ACOMEFTEMLi-ion batteryLiFePO(4)Phase map

More Related Videos

Obtaining 3D Chemical Maps by Energy Filtered Transmission Electron Microscopy Tomography
08:15

Obtaining 3D Chemical Maps by Energy Filtered Transmission Electron Microscopy Tomography

Published on: June 9, 2018

6.8K
In Depth Analyses of LEDs by a Combination of X-ray Computed Tomography CT and Light Microscopy LM Correlated with Scanning Electron Microscopy SEM
10:42

In Depth Analyses of LEDs by a Combination of X-ray Computed Tomography CT and Light Microscopy LM Correlated with Scanning Electron Microscopy SEM

Published on: June 16, 2016

9.8K

Related Experiment Videos

Last Updated: Mar 17, 2026

Analysis of Minerals Produced by hFOB 1.19 and Saos-2 Cells Using Transmission Electron Microscopy with Energy Dispersive X-ray Microanalysis
14:55

Analysis of Minerals Produced by hFOB 1.19 and Saos-2 Cells Using Transmission Electron Microscopy with Energy Dispersive X-ray Microanalysis

Published on: June 24, 2018

9.8K
Obtaining 3D Chemical Maps by Energy Filtered Transmission Electron Microscopy Tomography
08:15

Obtaining 3D Chemical Maps by Energy Filtered Transmission Electron Microscopy Tomography

Published on: June 9, 2018

6.8K
In Depth Analyses of LEDs by a Combination of X-ray Computed Tomography CT and Light Microscopy LM Correlated with Scanning Electron Microscopy SEM
10:42

In Depth Analyses of LEDs by a Combination of X-ray Computed Tomography CT and Light Microscopy LM Correlated with Scanning Electron Microscopy SEM

Published on: June 16, 2016

9.8K

Area of Science:

  • Materials Science
  • Electrochemistry
  • Microscopy

Background:

  • Lithium iron phosphate (LFP) is a key cathode material for next-generation lithium-ion batteries.
  • Accurate mapping of lithium distribution in LFP is challenging due to lithium's weak interaction with electrons.

Purpose of the Study:

  • To compare and validate different Transmission Electron Microscopy (TEM) techniques for mapping lithium distribution in LFP.
  • To assess the reliability and practical aspects of spectroscopic and STEM diffraction methods for battery material analysis.

Main Methods:

  • Utilized energy-filtered TEM (EF-TEM) across low-loss to core-loss energy ranges.
  • Employed automated crystal orientation mapping (ACOM) in STEM mode.
  • Directly compared results from multiple techniques on the same sample location.

Main Results:

  • All applied TEM methods (EF-TEM and ACOM-STEM) yielded consistent lithium distribution maps.
  • The agreement validates the reliability of both spectroscopic and STEM diffraction phase mapping techniques.
  • ACOM-STEM provided additional crystallographic insights into LFP/FP interfaces.

Conclusions:

  • Spectroscopic and STEM diffraction TEM are reliable for mapping lithium distribution in LFP.
  • Method comparison provides crucial data for designing in-situ experiments with beam-sensitive battery materials.
  • ACOM-STEM offers deeper understanding of LFP/FP interface properties, including phase boundary orientations.