Investigating long noncoding RNAs using animal models | JoVE Visualize

Area of Science:

Genomics
Molecular Biology
Developmental Biology

Background:

The number of identified long noncoding RNAs (lncRNAs) has rapidly increased, yet their functional roles are not well understood.
While cell cultures offer insights into molecular functions, animal models provide a more comprehensive context for investigating lncRNA impacts on phenotypes.
Previous research indicates lncRNAs influence a wide range of biological processes and phenotypes, from development to organismal viability.

Purpose of the Study:

To review the diverse array of animal models utilized for studying long noncoding RNA (lncRNA) function.
To discuss methodologies for profiling lncRNA expression within animal models.
To evaluate experimental approaches for manipulating lncRNA function in vivo and to summarize observed phenotypes.

Main Methods:

Literature review of studies employing animal models to investigate lncRNA function.
Analysis of techniques for lncRNA expression profiling in various animal systems.
Evaluation of in vivo experimental strategies for lncRNA manipulation (e.g., knockout, knockdown, overexpression).
Compilation of reported phenotypes associated with lncRNA dysregulation in animal models.

Main Results:

Animal models offer unique advantages for studying lncRNA-associated phenotypes, ranging from subtle morphological changes to effects on viability.
Diverse animal models, including mice, zebrafish, and Drosophila, have been instrumental in uncovering lncRNA functions.
Experimental strategies for in vivo manipulation and expression profiling are advancing, enabling deeper functional characterization.

Conclusions:

Long noncoding RNAs (lncRNAs) represent a significant class of molecules with critical implications for health and disease.
Animal models are indispensable for elucidating the in vivo functions and phenotypic consequences of lncRNAs.
Despite the current limited number of studies, the role of lncRNAs in biological systems is increasingly recognized and warrants further investigation.