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Immunohistochemical techniques for the human inner ear.

Ivan A Lopez1, Gail Ishiyama2, Seiji Hosokawa1,3

  • 1Head and Neck Surgery Department, David Geffen School of Medicine at UCLA, 951794, 35-64 Rehab, 1000 Veteran Avenue, Los Angeles, CA, 90095, USA.

Histochemistry and Cell Biology
|August 3, 2016
PubMed
Summary

This review details immunohistochemical staining methods for the human inner ear, comparing frozen, paraffin, and celloidin sections. Microdissection of auditory and vestibular endorgans offers excellent preservation for studying protein expression in human temporal bone research.

Keywords:
Antigen retrievalArchival human temporal boneCelloidin-embedded sectionsCochleaCryosectionsImmunohistochemistryMicrodissectionParaffinVestibule

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Area of Science:

  • Oto-rhino-laryngology
  • Neuroscience
  • Cell Biology

Background:

  • Immunohistochemistry (IHC) is crucial for studying protein expression in the inner ear.
  • Traditional methods have limitations in preserving antigenicity in human temporal bone samples.
  • Microdissection techniques offer potential for improved analysis of inner ear tissues.

Purpose of the Study:

  • To review recent immunohistochemical staining methods for the human inner ear.
  • To evaluate the application of these methods in microdissected auditory and vestibular endorgans.
  • To compare the advantages and disadvantages of IHC across different embedding media.

Main Methods:

  • Formalin-fixed frozen, paraffin, and celloidin-embedded human inner ear sections were used.
  • Immunohistochemistry and immunofluorescence were applied to microdissected endorgans.
  • Antigen retrieval methods were employed for celloidin-embedded sections.

Main Results:

  • IHC in frozen and paraffin sections provides robust immunoreactive signals.
  • Microdissected whole endorgans show excellent preservation of morphology and antigenicity.
  • Celloidin-embedded sections, with antigen retrieval, allowed detection of several antigens.

Conclusions:

  • Frozen and paraffin sections are viable alternatives when celloidin embedding is unavailable.
  • Microdissection of inner ear endorgans enables detection of regional protein expression variations.
  • These IHC techniques applied to human temporal bone open new avenues for inner ear research.