Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

RNA-seq03:21

RNA-seq

12.4K
RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
12.4K
Modern Molecular Taxonomy01:29

Modern Molecular Taxonomy

815
Advancements in molecular biology have revolutionized the identification and characterization of bacteria, with multiple methods leveraging DNA sequencing for enhanced precision. As sequencing technologies improve and costs decline, these approaches are increasingly used in clinical, environmental, and evolutionary studies.Multilocus Sequence Typing (MLST) examines several housekeeping genes, essential chromosomal genes encoding cellular functions, to distinguish strains. Approximately...
815
Genomics02:02

Genomics

41.5K
Genomics is the science of genomes: it is the study of all the genetic material of an organism. In humans, the genome consists of information carried in 23 pairs of chromosomes in the nucleus, as well as mitochondrial DNA. In genomics, both coding and non-coding DNA is sequenced and analyzed. Genomics allows a better understanding of all living things, their evolution, and their diversity. It has a myriad of uses: for example, to build phylogenetic trees, to improve productivity and...
41.5K
Next-generation Sequencing03:00

Next-generation Sequencing

100.6K
The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features....
100.6K
Applications of Molecular Taxonomy01:20

Applications of Molecular Taxonomy

648
Molecular taxonomy has revolutionized the understanding and classification of bacteria, providing precise insights into their diversity, evolutionary relationships, and ecological roles. By utilizing molecular techniques such as DNA sequencing and fingerprinting, researchers have made significant strides in various fields related to bacterial studies.Resolving Taxonomic AmbiguitiesMolecular taxonomy has been instrumental in distinguishing closely related bacterial species initially thought to...
648
Ribosome Profiling02:24

Ribosome Profiling

4.3K
Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...
4.3K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The mitochondrial genome of <i>Carex pseudochinensis</i> H. Lév. & Vaniot, an endemic sedge in Korea.

Mitochondrial DNA. Part B, Resources·2025
Same author

Complete mitochondrial genome of <i>Cyclograpsus intermedius</i> Ortmann, 1894 (Crustacea: Decapoda: Grapsoidea) specimen collected in South Korea.

Mitochondrial DNA. Part B, Resources·2021
Same author

A chloroplast genome of <i>Forsythia saxatilis</i> (Nakai) Nakai, an endemic species in Korea.

Mitochondrial DNA. Part B, Resources·2020
Same author

Silicon Enhances Biomass and Grain Yield in an Ancient Crop Tef [<i>Eragrostis tef (Zucc.) Trotter</i>].

Frontiers in plant science·2020
Same author

Lipid Composition of Latex and Rubber Particles in <i>Hevea brasiliensis</i> and <i>Taraxacum kok-saghyz</i>.

Molecules (Basel, Switzerland)·2020
Same author

Prediction of Dhurrin Metabolism by Transcriptome and Metabolome Analyses in <i>Sorghum</i>.

Plants (Basel, Switzerland)·2020
Same journal

Corrigendum: Paenibacillus marinisediminis sp. nov., a bacterium isolated from marine sediment.

Journal of microbiology (Seoul, Korea)·2026
Same journal

16S-Pipeline: A comprehensive web-based platform for end-to-end 16S rRNA amplicon sequencing analysis.

Journal of microbiology (Seoul, Korea)·2026
Same journal

Aliikangiella litoralis sp. nov. and Aliikangiella aequoris sp. nov., isolated from coastal seawaters of the Yellow Sea.

Journal of microbiology (Seoul, Korea)·2026
Same journal

Delineated domain of VP2 capsid protein in H-1 parvovirus that determines susceptibility to human cancer cells.

Journal of microbiology (Seoul, Korea)·2026
Same journal

Revealing genetic variation of Actinobacillus pleuropneumoniae Korean isolates using whole genome sequence analysis.

Journal of microbiology (Seoul, Korea)·2026
Same journal

Crystal structure of Bcl-2 from lymphocystis disease virus 2 in complex with the BH3 domain of zebrafish BaxA.

Journal of microbiology (Seoul, Korea)·2026
See all related articles

Related Experiment Video

Updated: Mar 17, 2026

Analyzing Gene Expression from Marine Microbial Communities using Environmental Transcriptomics
13:51

Analyzing Gene Expression from Marine Microbial Communities using Environmental Transcriptomics

Published on: February 18, 2009

13.0K

On the study of microbial transcriptomes using second- and third-generation sequencing technologies.

Sang Chul Choi1

  • 1Department of Biology, Sungshin Women's University, Seoul, 01133, Republic of Korea. sangchulchoi@sungshin.ac.kr.

Journal of Microbiology (Seoul, Korea)
|August 3, 2016
PubMed
Summary
This summary is machine-generated.

Second-generation sequencing advanced microbial transcriptome studies, revealing gene expression and improving genome annotation. Third-generation sequencing promises further breakthroughs in microbial research.

Keywords:
Oxford Nanopore MinIONRNA-seqbioinformaticsdifferential expressionfunctional enrichmentlibrary preparationquality control

More Related Videos

Purifying the Impure: Sequencing Metagenomes and Metatranscriptomes from Complex Animal-associated Samples
11:23

Purifying the Impure: Sequencing Metagenomes and Metatranscriptomes from Complex Animal-associated Samples

Published on: December 22, 2014

37.8K
Next-generation Sequencing of 16S Ribosomal RNA Gene Amplicons
10:24

Next-generation Sequencing of 16S Ribosomal RNA Gene Amplicons

Published on: August 29, 2014

84.8K

Related Experiment Videos

Last Updated: Mar 17, 2026

Analyzing Gene Expression from Marine Microbial Communities using Environmental Transcriptomics
13:51

Analyzing Gene Expression from Marine Microbial Communities using Environmental Transcriptomics

Published on: February 18, 2009

13.0K
Purifying the Impure: Sequencing Metagenomes and Metatranscriptomes from Complex Animal-associated Samples
11:23

Purifying the Impure: Sequencing Metagenomes and Metatranscriptomes from Complex Animal-associated Samples

Published on: December 22, 2014

37.8K
Next-generation Sequencing of 16S Ribosomal RNA Gene Amplicons
10:24

Next-generation Sequencing of 16S Ribosomal RNA Gene Amplicons

Published on: August 29, 2014

84.8K

Area of Science:

  • Microbiology
  • Genomics
  • Molecular Biology

Background:

  • Second-generation sequencing (SGS) revolutionized microbial transcriptome analysis.
  • SGS enabled detailed studies of transcription start sites, antisense transcripts, and genome-wide gene expression.
  • These advancements significantly improved microbial genome annotation and functional research.

Purpose of the Study:

  • To review microbial transcriptomics studies utilizing second-generation sequencing (SGS) technology.
  • To discuss the future prospects of microbial transcriptomics with the advent of third-generation sequencing (TGS).

Main Methods:

  • Review of existing literature on microbial transcriptomics using SGS.
  • Analysis of the capabilities and potential applications of TGS, specifically Oxford Nanopore Technologies' MinION platform.

Main Results:

  • SGS has been instrumental in understanding microbial gene expression and regulation.
  • TGS offers new possibilities for comprehensive microbial transcriptome analysis.

Conclusions:

  • Microbial transcriptomics has greatly benefited from SGS.
  • Third-generation sequencing holds significant promise for future discoveries in microbial research.